Abundance of some skeletal muscle mitochondrial proteins is associated with increased blood serum insulin in bovine fetuses |
| |
Authors: | Beata Pajak Patrycja Pawlikowska Isabelle Cassar-Malek Brigitte Picard Jean-Franois Hocquette Arkadiusz Orzechowski |
| |
Institution: | a Mossakowski Medical Research Center, Polish Academy of Sciences, Pawinskiego 5, 02-106 Warsaw, Poland;b Cochin Institute, Department of Hematology, INSERM, U567, CNRS, UMR 8104, Paris Decartes University, UMR-S 8104, Paris, F-75014, France;c INRA, UR1213, Unité de Recherches sur les Herbivores, Equipe Croissance et Métabolisme du Muscle, Theix, 63122 Saint-Genès Champanelle, France;d Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776 Warsaw, Poland |
| |
Abstract: | The aim of this study was to investigate the evolution of the abundance of cytochrome oxidase c subunit IV (NCOIV) and beta subunit of ATP synthase (β-ATP) during the last third of gestation in bovine skeletal muscles. Semitendinosus, longissimus thoracis and rectus abdominis muscles were chosen for the immunoblotting of the respective protein levels. Muscle and blood samples from bovine fetuses of randomly selected breeds were collected at 180, 210, and 260 days post-conception (dpc). The muscle tissue expressions of NCOIV, β-ATP were compared to blood glucose and insulin. At 260 dpc, protein levels of NCOIV raised in skeletal muscles. Additionally, β-ATP in semitendinosus and longissimus thoracis were elevated and paralleled by higher concentrations of blood serum insulin. It corroborates our previous observations indicating that accelerated metabolic differentiation of bovine skeletal muscles is associated with elevated blood insulin and occurs during the last trimester of gestation. Our observations point to the connection between insulin-sensitivity and the molecular mechanisms of mitochondrial contribution to ontogenesis of skeletal muscles. |
| |
Keywords: | ATP synthase Bovine skeletal muscles Cytochrome-c oxidase Insulin Myogenesis Pregnancy |
本文献已被 ScienceDirect 等数据库收录! |
|