用大孔吸附树脂对洋蓟茎叶中绿原酸及洋蓟素分离纯化的研究

筛选大孔树脂分离纯化朝鲜蓟茎叶中绿原酸及洋蓟素的最佳工艺,为后续研究及工业化大生产提供指导。以大孔树脂对绿原酸及洋蓟素的吸附率和解吸附率为指标筛选树脂种类,以上样速度、上样液中绿原酸与洋蓟素的质量浓度为指标,考察不同条件下AB-8大孔树脂对洋蓟茎叶提取液中绿原酸及洋蓟素分离纯化;以固体物中绿原酸及洋蓟素的质量浓度为指标,确立洗脱用乙醇的浓度及洗脱方式;采用HPLC法测定绿原酸及洋蓟素的含量。试验结果表明,选择AB-8大孔树脂,最佳纯化工艺为上样液绿原酸与洋蓟素的质量浓度分别为0.62,0.42 mg/mL,上样速度为1 BV/h;最佳洗脱方式是:用3倍柱体积的水洗涤除杂,再分别用2倍柱体积10%和75%的乙醇依次洗脱,最后用1倍柱体积的纯水冲洗,收集洗脱液与水洗液。HPLC法测定绿原酸及洋蓟素的质量分数分别为5.5%,4.6%。AB-8大孔树脂对洋蓟茎叶中绿原酸及洋蓟素分离纯化的综合性能较好,适合于工业化大生产,并符合市售产品要求。

Separation and Purification of Chlorogenic Acid and Cynarin from the Cynara Scolymus L. Leaf and Stem with Macroporous Resin

A method for separation and purification of chlorogenic acid and cynarin from the Cynara scolymus L.leaf and stem with macroporous resin is studied.Using the purity of extracts of chlorogenic acid and cynarin from Cynara scolymus L.leaf and stem as indexes,three kinds of macroporous resin are used to study the separation and purification performance.The velocity,the concentration and the eluant effect of separation and purification performance in static and dynamic are studied.The result shows that AB-8 type resin is a good choice in separation and purification of chlorogenic acid and cynarin from the Cynara scolymus L.leaf and stem.The optimum conditions are that AB-8 type macroporous resin,the concentration of chlorogenic acid and cynarin in sample is 0.62 mg/mL and 0.42 mg/mL respectively.The velocity is equal to the volume of the resin per hour.The eluants are as flollows: 10% alcohol is used as the eluant and 2 times as the volume of the resin,then the eluant is 75% alcohol and 2 times as the volume of the resin,at last,using purity water and equal to the volume of the resin.The purity of chlorogenic acid and cynarin is 5.5% and 4.6% respectively.