猪饲料中有机胂类药物残留检测方法——高效液相色谱法

有机胂类药物是一类抗菌促生长药物,具有促生长、改善饲料效率的作用,并且对产蛋和猪毛色均有较好作用。但是长期使用含有有机胂砷制剂的饲料喂养动物,这些药物会残留于动物的器官、组织中,通过食物链危害人类健康。建立高效液相色谱法对猪饲料中4种有机胂类药物[对氨基苯胂酸(阿散酸)、3-硝基-4-羟基苯胂酸(洛克沙胂)、4-硝基硝苯胂酸(硝苯胂酸)、对脲基苯胂酸(卡巴胂)]的残留进行了检测。实验结果表明,饲料样品以甲醇和乙酸混合溶液为提取剂,CuSO4沉淀蛋白,通过采用高效液相色谱-紫外法检测,4种有机胂类药物线性范围为0.1~20μg/mL,定量限为5μg/g,平均回收率>80%,相对标准偏差<10%。该方法能同时检测猪饲料中4种有机胂类药物残留,前处理简单,回收率高,检出限低,可为饲料样品中有机胂类药物残留的监测与控制提供有效的检测方法。

Residue Determination of Organic Arsenic in Pig Feeds Using High Performance Liquid Chromatography

Organic arsenic are widely used as medicinal feed additives as antibacterial growth promoters for pig and chicken,which has good effect on producing eggs and keeping fair of pig.But due to their side-effect,there is an urgent need to develop comprehensive control measures to monitor organic arsenic.A high performance liquid chromatography method with UV detection has been established for simultaneous determination of four kinds of organic arsenic in pig feeds,including arsanilic acid,roxarsone,nitarsone and...