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| 利用正交设计优化百子莲农杆菌转化条件的初探 | |
| 引用本文： | 刘红梅,卓丽环,刘群录,徐有明. 利用正交设计优化百子莲农杆菌转化条件的初探[J]. 上海交通大学学报(农业科学版), 2011, 29(1): 62-67. DOI: 10.3969/J.ISSN.1671-9964.2011.01.012 |
| 作者姓名： | 刘红梅 卓丽环 刘群录 徐有明 |
| 作者单位： | 1. 华中农业大学,园艺与林学学院,武汉,271017 2. 上海农林职业技术学院,上海,201600 3. 上海交通大学,农业与生物学院,农业部都市农业(南方)重点试验室,上海,200240 |
| 基金项目： | 上海市科技兴农重点攻关项目[沪农科攻字(2006)第4~9号] |
| 摘 要： | 利用正交试验设计,以百子莲胚性愈伤组织为受体材料,通过GUS染色确定遗传转化率,对农杆菌介导的百子莲遗传转化体系进行了优化。结果表明,百子莲遗传转化优化体系为：愈伤组织预培养7 d、菌液浓度（OD600）为0.9、侵染时间为5 min、预培养培养基中乙酰丁香酮（AS）的浓度为50μmol.L-1、侵染菌液中乙酰丁香酮（AS）的浓度为100μmol.L-1。在优化的转化条件下,可提高农杆菌EHA105菌株介导的百子莲愈伤组织遗传转化率。 |
| 关 键 词： | 百子莲 农杆菌 转化条件 正交设计 |
Preliminary Study on Optimization in Agrobacterium-Mediated Transformation System for Agapanthus africanus by Orthogonal Design |
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| LIU Hong-mei,ZHUO Li-huan,LIU Qun-lu,XU You-ming. Preliminary Study on Optimization in Agrobacterium-Mediated Transformation System for Agapanthus africanus by Orthogonal Design[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 2011, 29(1): 62-67. DOI: 10.3969/J.ISSN.1671-9964.2011.01.012 | |
| Authors: | LIU Hong-mei ZHUO Li-huan LIU Qun-lu XU You-ming |
| Affiliation: | LIU Hong-mei1,ZHUO Li-huan2,LIU Qun-lu3,XU You-ming1(1.College of Horticulture and Forestry Sciences,HuaZhong Agriculture University,Wuhan 271017,China,2.Shanghai Vocational and Technical College of Agriculture and Forestry,Shanghai 201600,3.Key Laboratory of Urban Agriculture(South) of Ministry of Agriculture,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China) |
| Abstract: | In this article,the agrobacteriu-mediated genetic transformation system of Agapanthus africanus was optimized through orthogonal design using the Agapanthus africanus embryogenic calli as materials.The genetic transformation percentage was determined by GUS staining.The results showed that the optimized system included：7 d pre-culturing,bacteria cell density（OD600） 0.9,5 min infection incubation,50 μmol·L-1 acetosyringone（AS） in the pre-culture media,100 μmol·L-1 acetosyringone（AS） in the infection culture media.Under the optimized conditions,the genetic transformation of Agapanthus africanus embryogenic calli mediated by EHA105 strain increased. |
| Keywords: | Agapanthus africanus Agrobacterium tumefactions transformation conditions orthogonal design |
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