| 杨树烂皮病菌颉颃菌株的初步筛选与分子鉴定 |
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| 引用本文： | 徐明,吴小芹. 杨树烂皮病菌颉颃菌株的初步筛选与分子鉴定[J]. 江苏林业科技, 2012, 39(5): 1-6,30 |
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| 作者姓名： | 徐明 吴小芹 |
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| 作者单位： | 1. 江苏省林业科学研究院,江苏南京211153 南京林业大学,江苏南京210037
2. 南京林业大学,江苏南京,210037 |
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| 摘 要： | 对杨树烂皮病菌的平板对峙试验结果表明,6种颉颃真菌中木霉的抑菌率最高为51%,而颉颃细菌4个菌株的抑菌率均在60%左右,说明颉颃细菌的抑菌效果明显高于颉颃真菌;对B137等4株颉颃细菌的分子鉴定认为,1株为枯草芽孢杆菌(Bacillus subtilis),2株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),还有1株为苏云金杆菌(Bacillus thuringiensis,Bt);解淀粉芽孢杆菌和苏云金杆菌对杨树烂皮病菌有较好的颉颃效果。
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| 关 键 词： | 杨树烂皮病 分子鉴定 生物防治 |
The Preliminary studies of biological control of Cytospora canker of poplar and molecular identification |
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| XU Ming,WU Xiao-qin. The Preliminary studies of biological control of Cytospora canker of poplar and molecular identification[J]. Journal of Jiangsu Forestry Science & Technology, 2012, 39(5): 1-6,30 |
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| Authors: | XU Ming WU Xiao-qin |
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| Affiliation: | 1.Forestry Academy of Jiangsu Province,Nanjing 211153,China;2.Nanjing Forestry University,Nanjing 210037,China) |
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| Abstract: | The test of the biological control of Cytospora canker of poplar indoor found that the maximum teriostasic rate of the antagonistic and fungi reached 51% and the average teriostasic rate of the bacteria tested was 60%.The antagonistic bacteria had better effects than antagonistic fungi.The molecular identification found that 4 antngonistic bacteria tested contained 1 strain of Bacillus subtilis,2 strains of B.amyloliquefaciens and 1 strain of B.thuringiensis,which were isolated from the poplar with good antagonistic effects to Cytospora.These strains provide a theoretical basis for the development and production of biological medicine in the future. |
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| Keywords: | Cytospora Canker of poplar Molecular identification Biological Control |
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