Technical note: Development of an enzyme-linked immunosorbent assay for the determination of florfenicol and thiamphenicol in swine feed |
| |
| Authors: | Luo P J Jiang W X Chen X Shen J Z Wu Y N |
| |
| Institution: | Key Laboratory of Chemical Safety and Health, National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, People's Republic of China. |
| |
| Abstract: | One polyclonal antibody against florfenicol and thiamphenicol was produced and a competitive ELISA was developed for the detection of florfenicol and thiamphenicol in swine feed. The ELISA gave a 50% inhibiting concentration of 1.02 ng/mL for florfenicol. For swine feed fortified with 0.05 to 3.0 mg/kg, the interassay recoveries of florfenicol and thiamphenicol ranged from 86.4 to 118.6%, whereas intraassay recoveries of both drug ranged from 90.1 to 126.5% with less than 15% CV. Results obtained from HPLC-tandem mass spectrometry indicated this ELISA procedure could be used as a convenient method for rapid screening of florfenicol and thiamphenicol in swine feed. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
