Identification of flea species using MALDI-TOF/MS |
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| Authors: | Amina Yssouf Cristina Socolovschi Hamza Leulmi Tahar Kernif Idir Bitam Gilles Audoly Lionel Almeras Didier Raoult Philippe Parola |
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| Institution: | 1. Aix Marseille Université, Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, CNRS 7278, IRD 198, Inserm 1095, WHO Collaborative Center for Rickettsioses and Other Arthropod-Borne Bacterial Diseases, Faculté de Médecine, 27 bd Jean Moulin, 13385 Marseille cedex 5, France;2. Institut Pasteur d’Algérie, 1 Rue du Docteur Laveran, Hamma, Alger, Algeria;3. Laboratoire Biodiversité et Environnement: Interactions, Génomes, Faculté des Sciences Biologique, Université des Sciences et de la Technologie Houari Boumediene, Alger, Algeria |
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| Abstract: | In the present study, a molecular proteomics (MALDI-TOF/MS) approach was used as a tool for identifying flea vectors. We measured the MS spectra from 38 flea specimens of 5 species including Ctenocephalides felis, Ctenocephalides canis, Archaeopsylla erinacei, Xenopsylla cheopis and Stenoponia tripectinata. A blind test performed with 24 specimens from species included in a library spectral database confirmed that MALDI-TOF/MS is an effective tool for discriminating flea species. Although fresh and 70% ethanol-conserved samples subjected to MALDI-TOF/MS in blind tests were correctly classified, only MS spectra of quality from fresh specimens were sufficient for accurate and significant identification. A cluster analysis highlighted that the MALDI Biotyper can be used for studying the phylogeny of fleas. |
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| Keywords: | MALDI-TOF-mass spectrometry Fleas Identification |
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