华南三地观光木遗传多样性的RAPD分析

报道了广东南昆山和始兴都亨、广西靖西三地的观光木Tsoongiodendron odorum居群基因多态性研究的初步结果，应用随机扩增多态DNA（RAPD）分子标记方法，从108个10个碱基的随机引物中获得了18个多态性标记，在3个居群共42个样株中，共检测到78个位点，其中多态位点66个，占84.62%，显示该物种的基因多样性较高，聚类分析结果表明，样品可分为3个居群；广东境内的2个居群间相似性较高，相似性系数达0.832,广西与广东都亨和南昆山2个居群间的相似性较低，相似性系数分别为0.772和0.768，南昆山居群内的基因多样性最高，广东始兴都亨居群的基因多样性最低。广西居群居中。

Comparison of Genetic Diversity of Tsoongiodendron odorum in Southern China by RAPD Markers

The preliminary results on genetic diversity of Tsoongiodendron odorum at three sites of southern China were described. Eighteen 10 mer primers selected from 108 were applied for RAPD analysis. Seventy-eight genes were obtained from 42 leaf samples from the three populations, 66 of them (84.62% of total genes) were polymorphic. The result of cluster analysis showed that the population of Nankunshan of Guangdong province had the highest genetic diversity (3.074), the second was Jingxi of Guangxi province(2.389), the least was Duheng of Guangdong province (2 195). The similarity index between Nankunshan and Duheng in Guangdong province was the highest (0.832), that of Jingxi and Duheng was 0 772, and the similarty index between Jingxi and Nankunshan was 0 768.