黑蜣(Bess beetle)纤维素酶的组分及其特性分析

以30日龄的黑蜣幼虫为材料,对其整体匀浆后组织液中的纤维素酶组成及活性进行了分析。结果表明:黑蜣体内存在内切β-1,4-葡聚糖酶(Cx酶)、β-葡萄糖苷酶;β-葡萄糖苷酶和Cx酶的最适宜温度为分别为40℃和50℃;Cx酶和β-葡萄糖苷酶分别在pH值为6.4和5.2时具有最大的活力;β-葡萄糖苷酶和Cx酶的最适宜反应时间为80 min。Cx酶具有比β-葡萄糖苷酶更强的温度适应性,在30～50℃温度范围内可维持最大酶活力的66.20%。

Analysis on Characterization and Constitution of Cellulase in Bess beetle

The property of major cellulases from the whole body of Bess beetle larvae(30 d)had been characterized.The results showed that the Bess beetle contacted Cx(endo-β-1,4-endoglucanase)and exo-β-1,4-glucanase.The optimal temperatures for exo-β-1,4-glucanase was 40 ℃,and Cx(endo-β-1,4-endoglucanase) was 50 ℃.Endo-β-1,4-glucanase was optimally active at pH value 6.4,while the optimal activity of the exo-β-1,4-glucanase occurred at pH value 5.2.The optimal active time for exo-β-1,4-glucanaseis and endo-β-1,4-endoglucanase was 80 min.The endo-glucanase was active with a wider range of temperature than exo-β-1,4-glucanaseis,the levels of activity from 30 ℃ to 50 ℃ were over 66.20%.