| 检测鸡蛋中沙门氏菌的LAMP方法的建立及初步应用 |
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| 引用本文： | 唐梦君,周生,张小燕,顾荣,蒲俊华,葛庆联,高玉时.检测鸡蛋中沙门氏菌的LAMP方法的建立及初步应用[J].安徽农业大学学报,2011,38(1):43-47. |
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| 作者姓名： | 唐梦君 周生 张小燕 顾荣 蒲俊华 葛庆联 高玉时 |
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| 作者单位： | 农业部家禽品质监督检验测试中心,扬州,225003;中国农业科学院家禽研究所,扬州,225003;中国农业科学院家禽研究所,扬州,225003 |
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| 基金项目： | 江苏省科技创新与成果转化专项引导资金(NO.BM2009908); 国家蛋鸡产业体系(NYCYTX-41-2B)共同资助 |
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| 摘 要： | 根据GenBank上公布的沙门氏菌invA基因序列中的保守区域,设计一套环介导等温扩增(LAMP)引物,将其用于沙门氏菌的检测,结果成功地扩增出特异性的梯形条带。LAMP方法检测沙门氏菌纯培养的灵敏度为1.04×102 cfu.mL-1,对11株不同细菌进行LAMP检测,仅沙门氏菌获得阳性结果。应用该方法对扬州市场上销售的鸡蛋进行沙门氏菌检测,检测结果与传统培养方法相符合。因此,LAMP方法检测沙门氏菌具有灵敏度高,特异性强,耗时短,方法简便等特点,有望发展成为快速检测鸡蛋中沙门氏菌的有效手段。
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| 关 键 词： | 沙门氏菌 环介导等温扩增技术 鸡蛋 |
Loop-mediated isothermal amplification assay for rapid detection of Salmonella spp. |
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| TANG Meng-jun,ZHOU Sheng,ZHANG Xiao-yan,GU Rong,PU Jun-hu,GE Qing-lian and GAO Yu-shi.Loop-mediated isothermal amplification assay for rapid detection of Salmonella spp.[J].Journal of Anhui Agricultural University,2011,38(1):43-47. |
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| Authors: | TANG Meng-jun ZHOU Sheng ZHANG Xiao-yan GU Rong PU Jun-hu GE Qing-lian and GAO Yu-shi |
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| Institution: | TANG Meng-jun1,2,ZHOU Sheng2,ZHANG Xiao-yan1,GU Rong1,PU Jun-hua1,GE Qing-lian1,GAO Yu-shi1,2(1.Quality Inspection Center for Poultry of Ministry of Agriculture,Yangzhou 225003,2.Poultry Insitute,Chinese Academy of Agricultural Sciences,Yangzhou 225003) |
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| Abstract: | Salmonella spp.is an important kind of food-borne pathogenic bacteria which can cause human and animal disease.Salmonella invA gene is known as a major virulence gene.According to published Salmonella invA gene sequence in GenBank,we designed one sets of specific loop-mediated isothermal amplification primers and developed a novel and highly specific loop-mediated isothermal amplification assay for the sensitive and rapid detection of Salmonella spp.The assay correctly identified Salmonella spp.,but did not... |
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| Keywords: | Salmonella spp Loop-mediated isothermal amplification egg |
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