| 家蚕人工授精关键技术的研究 |
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| 引用本文： | 张业顺,张国政,韦亚东,夏定国.家蚕人工授精关键技术的研究[J].农业科学与技术,2009,10(4):167-170. |
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| 作者姓名： | 张业顺 张国政 韦亚东 夏定国 |
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| 作者单位： | 张业顺,张国政(江苏科技大学,江苏镇江,212003;中国农业科学院农业部蚕桑遗传改良重点实验室,江苏镇江,212018);韦亚东,夏定国(中国农业科学院农业部蚕桑遗传改良重点实验室,江苏镇江,212018) |
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| 摘 要： | 1材料与方法
1．1材料家蚕品种为菁松、皓月，由该研究室提供；解剖镜为OLYMPUS SZ51；精子细胞采集所用器材均为自制。Penicillin—Streptomycin和胰蛋白酶购自Invitrogen公司。TC-100培养基购自Sigma公司。
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| 关 键 词： | 家蚕品种 人工授精 OLYMPUS Sigma公司 技术 精子细胞 胰蛋白酶 研究室 |
Study on Key Technique of Artificial Insemination for Silkworm, Bombyx mori |
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| ZHANG Ye-shun,ZHANG Guo-zheng.,WEI Ya-dong,XIA Ding-guo.Study on Key Technique of Artificial Insemination for Silkworm, Bombyx mori[J].Agricultural Science ＆ Technology,2009,10(4):167-170. |
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| Authors: | ZHANG Ye-shun ZHANG Guo-zheng WEI Ya-dong XIA Ding-guo |
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| Institution: | 1. Jiangsu University of Science and Technology, Zhenjiang 212003; 2. Key Laboratory of Silk Mulberry Genetic Improvement, Chinese Academy of Agricultural Sciences, Zhenjiang 212018) |
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| Abstract: | Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extruding and centrifugal method, and then the semen was injected into other virgin moths with trypsinase. Result] A high-effective collection technology of spermatids from silkworm was established successfully, 50 μl semen could be collected by only one person in each hour. The survival rate of spermatids was over 80% in vito after collected from bursa copulatrix, while the obtained semen was quite pure and the average fertilization rate of silkworm was 76,5%. Conclusion] The establishment of high-effective semen extraction technique of silkworm provides the technical basis for studies on other related techniques for silkworm sperm. |
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| Keywords: | Bombyx mori Artificial insemination Key technique |
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