|
|||||
|
|
| 甲基对硫磷压电免疫传感技术的初步探讨 | |
| 引用本文： | 卢澄,潘家荣,高志贤,姚伟.甲基对硫磷压电免疫传感技术的初步探讨[J].核农学报,2008,22(3):334-337. |
| 作者姓名： | 卢澄 潘家荣 高志贤 姚伟 |
| 作者单位： | 1. 中国农业科学院农产品加工研究所,北京,100193;天津军事医学科学院卫生学环境医学研究所,天津,300050 2. 中国农业科学院农产品加工研究所,北京,100193 3. 天津军事医学科学院卫生学环境医学研究所,天津,300050 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 分别利用蛋白A法和巯基自组装膜法将抗体固定在压电石英晶体金电极上,结果表明巯基自组装膜法效果更好。在利用巯基自组装膜法固定甲基对硫磷抗体的基础上,初步建立了基于直接法和竞争法的甲基对硫磷压电免疫传感技术并对两种技术作了比较,结果表明,基于竞争法的压电免疫传感技术对对硫磷抗原抗体反应的响应频移值较大,可提高检测灵敏度,降低检测限。 |
| 关 键 词： | 压电免疫传感技术 甲基对硫磷 巯基自组装膜法 竞争法 直接法 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
PRELIMINARY STUDY ON A NOVEL PIEZOELECTRIC IMMUNOSENSORS FOR DETECTION OF METHYL PARATHION |
|
| LU Cheng,PAN Jia-rong,GAO Zhi-xian,YAO Wei.PRELIMINARY STUDY ON A NOVEL PIEZOELECTRIC IMMUNOSENSORS FOR DETECTION OF METHYL PARATHION[J].Acta Agriculturae Nucleatae Sinica,2008,22(3):334-337. | |
| Authors: | LU Cheng PAN Jia-rong GAO Zhi-xian YAO Wei |
| Abstract: | Two antibody immobilization methods,immunosensory techniques based on direct and competitive methods for detection of methyl parathion,were compared in order to develop a simple,cheap,rapid,online-detectable and mass-response-based piezoelectric immunosensor.The results showed that the anti-methyl parathion polyclonal antibody was better immobilized on the golden electrode of the piezoelectric quartz crystals with sulfhydryl self-assembled layer than SPA method.Compared with direct method,piezoelectric immunosensor based on competitive method for methyl parathion detection was more sensitive due to larger frequency shift obtained. |
| Keywords: | piezoelectric immunosensor methyl parathion sulfhydryl self-assembled layer competitive detection direct method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《核农学报》浏览原始摘要信息 | |
| 点击此处可从《核农学报》下载免费的PDF全文 | |