Community composition of ammonia-oxidizing bacteria in the rhizosphere of intercropped wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.), maize (<Emphasis Type="Italic">Zea mays</Emphasis> L.), and faba bean (<Emphasis Type="Italic">Vicia faba</Emphasis> L.) |
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Authors: | Y N Song P Marschner L Li X G Bao J H Sun F S Zhang |
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Institution: | (1) Key Lab of Plant and Soil Interactions, MOE, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100094, China;(2) Soil and Land Systems, School of Earth and Environmental Sciences, University of Adelaide, DP 636, SA 5005 Adelaide, Australia;(3) Institute of Soils and Fertilizers, Gansu Academy of Agriculture Sciences, Lanzhou, 730000, China;(4) Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, China |
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Abstract: | Cereal/cereal and cereal/legume intercropping systems are popular in the north, northwest, and southwest of China and often
result in yield increases compared to monocropping. Rhizosphere interactions may play a significant role in the yield increases,
particularly with respect to nutrient availability. The aim of this study was to investigate the effects of intercropping
on N availability and community composition of ammonia-oxidizing bacteria in the rhizosphere of wheat, maize, and faba bean
at different growth stages. Denaturing gradient gel electrophoresis (DGGE) based on 16S rRNA genes was used to analyze the
community composition of bacterial ammonia oxidizers belonging to β-proteobacteria. The results showed that intercropping
with faba bean significantly increased nitrate concentrations in the rhizosphere of wheat and maize at the second sampling
time (20 June) compared to monocropping or intercropping between maize and wheat. Intercropping significantly affected the
community composition of ammonia-oxidizing bacteria in the rhizosphere compared to monocropping, and the effects were most
pronounced in the maize/faba bean and wheat/maize intercropping systems when faba bean and wheat were at anthesis and maize
was in seedling stage. In wheat/faba bean intercropping, the effects of intercropping on community composition of ammonia-oxidizing
bacteria were less pronounced at the seedling stage of the two species but were significant at anthesis. |
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Keywords: | Ammonia-oxidizing bacteria Community composition Intercropping PCR-DGGE Rhizosphere |
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