橡胶丛枝病分子检测研究

感染橡胶丛枝病的长春花（菟丝子桥接），通过提取其总DNA，以植原体（phytoplasma）特异引物，进行PCR扩增，结果得到860bp的DNA片断，片断的大小与设计的相符。而健康长春花扩增得不到此片断。同样，将感染丛枝病的橡胶树总DNA，进行PCR扩增，结果与上述相同。该860bp片断经地高辛标记，核酸杂交结果显示，感染丛枝病的橡胶树阳性反应强烈，健康橡胶树仅有微弱反应。

Detection of Rubber Witches'''' Broom Disease with PCR and Nucleotide Hybridization

A novel method in which PCR and DNA hybridization techniques were utilized was established for detecting rubber witches' broom disease associated with phytoplasma. The total DNA extracted from infected periwinkle and diseased rubbers were amplified using special oligo nucleotide primers. The product of PCR was 860 hp fragment. The size of the fragment was consistent with the distance between the two primers in the sequence of the Oenothera phytoplasma (863 bp). The template DNA from healthy periwinkle and rubber couldn't amplified the 860 bp fragment. This 860 bp fragment was used as probe with Dig labeling and dot blot hybridization. The results showed that the DNA of diseased rubber could be strongly hybridized with this fragment.