| 用AFLP分子标记鉴定冬枣自然授粉实生后代杂种的研究 |
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| 引用本文： | 鹿金颖,毛永民,申莲英,彭士琪,刘敏.用AFLP分子标记鉴定冬枣自然授粉实生后代杂种的研究[J].园艺学报,2005,32(4):680-684. |
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| 作者姓名： | 鹿金颖 毛永民 申莲英 彭士琪 刘敏 |
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| 作者单位： | (1 中国科学院遗传与发育生物学研究所, 北京100101; 2 河北农业大学中国枣研究中心, 保定071001) |
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| 基金项目： | 国家自然科学基金项目(30370994) |
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| 摘 要： | 应用AFLP技术对65株冬枣自然授粉实生后代进行杂种鉴定, 从81对EcoRⅠ和MseⅠ选择性引物中筛选出12对带型清晰且多态性较高的引物。用这12对引物共扩增出517条谱带, 其中多态性带376条, 多态性百分率为72.7%。共扩增出金丝小枣特征带10条, 鉴定出34株冬枣×金丝小枣杂交实生苗;扩增出尖枣特征带7条, 鉴定出冬枣×尖枣杂交实生苗15株; 不能确定的有16株。
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| 关 键 词： | 枣 实生后代 AFLP标记 杂种鉴定 |
| 文章编号： | 0513-353X（2005）04-0680-04 |
| 收稿时间： | 2005-02-01 |
| 修稿时间： | 2005-02-012005-06-06 |
Application of AFLP Markers for Identification of Hybrids from Open Pollinated Dongzao(Zizyphus jujuba Mill.) Progenies |
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| Lu Jinying,Mao Yongmin,Shen Lianying,Peng Shiqi,Liu Min.Application of AFLP Markers for Identification of Hybrids from Open Pollinated Dongzao(Zizyphus jujuba Mill.) Progenies[J].Acta Horticulturae Sinica,2005,32(4):680-684. |
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| Authors: | Lu Jinying Mao Yongmin Shen Lianying Peng Shiqi Liu Min |
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| Institution: | (1 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 2 Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding 071001, China) |
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| Abstract: | The 65 open-pollinated ‘Dongzao’ ( Zizyphus jujuba Mill. ) progenies were detected with AFLP technique. Twelve pairs of primers which amplified distinct and polymorphic segments were selected from 81 pairs of EcoRⅠand MseⅠ primers. Then twelve pairs of primers were used in the AFLP analysis. Altogether 517 bands were p roduced among which 376 bands were polymorphic ones with the percentage of polymorphism being 72.7%. Ten markers were present only in ‘Jinsi Xiaozao’. Thirty-four hybrids from‘Dongzao’בJinsi Xiaozao’were identificated. Seven markers were present only in ‘Jianzao’. Fifteen hybrids from ‘Dongzao’בJ ianzao’were identificated. Sixteen plants were not confirmed. |
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| Keywords: | Chinese jujube Progeny AFLP marker Identification of hybrid |
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