| 南瓜组织培养再生体系的研究 |
| |
| 引用本文： | 张卫华,孙小镭,王志峰,曹齐卫,刘文宝. 南瓜组织培养再生体系的研究[J]. 山东农业科学, 2006, 0(6): 4-6 |
| |
| 作者姓名： | 张卫华 孙小镭 王志峰 曹齐卫 刘文宝 |
| |
| 作者单位： | 1. 山东省农业科学院蔬菜研究所,山东,济南,250100
2. 山东农业科技开发总公司,山东,济南,250100 |
| |
| 摘 要： | 以黑籽南瓜和白籽南瓜为试材,研究了苗龄、激素配比、外植体、芽诱导时间以及基因型对再生频率的影响。结果表明,以3~5天苗龄的南瓜子叶为外植体,在MS+6-BA 1 mg/L+Ades 20 mg/L的诱导培养基上诱导10天后,转入MS+GA30.5 mg/L的芽伸长培养基,不同基因型南瓜均能获得较高的植株再生率,每个外植体成苗数在2.5个以上。从接种到再生苗移栽仅需4~5周时间。
|
| 关 键 词： | 南瓜 子叶 外植体 植株再生 |
| 文章编号： | 1001-4942(2006)06-0004-03 |
| 修稿时间： | 2006-08-03 |
Study on the High Efficient Tissue Culture System of Pumpkin |
| |
| ZHANG Wei-hua,SUN Xiao-lei,WANG Zhi-feng,CAO Qi-wei,LIU Wen-bao. Study on the High Efficient Tissue Culture System of Pumpkin[J]. Shandong Agricultural Sciences, 2006, 0(6): 4-6 |
| |
| Authors: | ZHANG Wei-hua SUN Xiao-lei WANG Zhi-feng CAO Qi-wei LIU Wen-bao |
| |
| Abstract: | Effects of seedling age,the hormone combination,different explants,the uime of shoot induction and different genotypes on pumpkin tissue culture efficiency were studied.The results showed that: when the pumpkin cotyledons from 3～5 days seedling were induced in the medium MS+6-BA 1 mg/L+Ades 20 mg/L for about 10 days,and then transferred into shoot elongation medium,MS+GA_3 0.5 mg/L,more plantlets could regenerate rapidly from different genotype explants.The time from cotyledon subculturing to plantlet transplanting needed only 4～5 weeks. |
| |
| Keywords: | Pumpkin Cotyledon Explants Plantlet regeneration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
