| 中国北方冬麦区主栽品种醇溶蛋白指纹图谱数据库的建立 |
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| 引用本文： | 郎明林,卢少源,等.中国北方冬麦区主栽品种醇溶蛋白指纹图谱数据库的建立[J].中国农业科学,2002,35(3):238-244. |
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| 作者姓名： | 郎明林 卢少源 |
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| 作者单位： | [1]中国科学院研究生院生物学部，北京100039 [2]河北农业大学生命科学学院，保定071001 |
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| 基金项目： | 国家自然科学基金资助项目 (3 9170 488) |
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| 摘 要： | 结合CAWGES软件，用改良的pH3.2 A-PAGE技术绘制并构建了我国北方冬麦区建国后不同时期的主栽品种及其部分骨干亲本共68个品种的标准麦醇溶蛋白指纹图谱及其数据库。并利用数据库在图谱分析、品种鉴定及血缘关系研究等方面进行了应用探讨，为建立小麦核心种质及品种鉴别提供了有效手段。
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| 关 键 词： | 冬小麦 主栽品种 醇溶蛋白 指纹图谱数据库 APAGE |
| 修稿时间： | 2001年1月5日 |
Construction of Gliadin Fingerprints Database of the Main Wheat Cultivars Grown in North China |
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| LANG Ming lin ,LU Shao yuan ,ZHANG Rong zhi.Construction of Gliadin Fingerprints Database of the Main Wheat Cultivars Grown in North China[J].Scientia Agricultura Sinica,2002,35(3):238-244. |
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| Authors: | LANG Ming lin LU Shao yuan ZHANG Rong zhi |
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| Institution: | LANG Ming lin 1,2,LU Shao yuan 2,ZHANG Rong zhi 2 |
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| Abstract: | The standard gliadin fingerprints and their database of 68 main cultivars and a part of their backbone parents, which were grown in North China since the 1950's,were constructed by using CAWGES software and an improved method of pH3 2 A PAGE. In the meantime, investigations were made on the utilization of the database in the area of gliadin fingerprints analysis, cultivar identification and analysis of genetic relationship. The results showed that it provided an effective method for building core germplasm of wheat and conducting variety identification. |
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| Keywords: | Winter wheat Main Cultivars Gliadin Fingerprints database A PAGE |
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