| 反向遗传技术在猪繁殖与呼吸综合征研究中的应用 |
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| 引用本文： | 谷元兴 王猛 张杰 刘永生. 反向遗传技术在猪繁殖与呼吸综合征研究中的应用[J]. 中国农学通报, 2011, 27(7): 364-368 |
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| 作者姓名： | 谷元兴 王猛 张杰 刘永生 |
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| 作者单位： | 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室,农业部兽医公共卫生重点开放实验室,兰州,730046 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 猪繁殖与呼吸综合征（PRRS）由猪繁殖与呼吸综合征病毒（PRRSV）引起，严重危害全世界养猪业。PRRSV与宿主细胞的相互作用、致病机理、其基因组、蛋白质对致病性的影响等仍是未解之谜。反向遗传学技术为进一步研究PRRSV提供了技术支撑。本文从病毒拯救、疫苗研制、分子病原学诊断等方面综述了反向遗传学在PRRS研究中的应用。
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| 关 键 词： | 变叶海棠 变叶海棠 生物学特性 遗传多样性 抗逆性 开发前景 |
| 收稿时间： | 2010-09-03 |
| 修稿时间： | 2010-10-11 |
Application of Reverse Genetics Manipulation in the Research of Porcine Reproductive and Respiratory Syndrome |
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| Gu Yuanxing,Wang Meng,Uu Yongsheng,Zhang Jie. Application of Reverse Genetics Manipulation in the Research of Porcine Reproductive and Respiratory Syndrome[J]. Chinese Agricultural Science Bulletin, 2011, 27(7): 364-368 |
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| Authors: | Gu Yuanxing Wang Meng Uu Yongsheng Zhang Jie |
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| Affiliation: | Gu Yuanxing,Wang Meng,Liu Yongsheng,Zhang Jie (Key laboratory of Veterinary Public Health of Agriculture,Key Laboratory of Animal Virology of Ministry of Agriculture,State Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046) |
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| Abstract: | Porcine reproductive and respiratory syndrome (PRRS),caused by porcine reproductive and respiratory syndrome virus (PRRSV),is considered one of the most important contagious diseases in swine industry today and responsible for significant economic losses worldwide.Reverse genetics manipulation has been used extensively in variety of fields of life science for several decades,which supported us a powerful method to explore organisms.This paper mainly summarized the application and perspective of reverse gene... |
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| Keywords: | reverse genetics manipulation virus rescue RNA virus PRRS applications |
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