魔芋甘露寡糖对三黄鸡免疫功能及盲肠菌群的影响

为探明甘露寡糖对三黄鸡免疫功能和盲肠菌群的影响及替代抗生素的可行性,选用1日龄三黄鸡216只,随机分为 6 组, 每组3 个重复, 每重复12 只鸡, 分别饲喂6 种不同日粮, 自由采食和饮水，第Ⅰ 组为空白对照组, 第Ⅱ，Ⅲ，Ⅳ，Ⅴ，Ⅵ组为试验组, 在基础日粮的基础上分别添加600 mg/kg林可霉素, 1 g/kg甘露寡糖，2 g/kg甘露寡糖，4 g/kg甘露寡糖和8 g/kg甘露寡糖。结果表明：（1）添加4 g/kg甘露寡糖，可显著提高胸腺指数(P<0.05)，有提高脾脏和法氏囊指数的趋势，但差异不显著(P>0.05)；（2）饲料中添加600 mg/kg林可霉素或4 g/kg甘露寡糖，均能显著提高血清溶菌酶活性(P<0.05)，而8 g/kg甘露寡糖组血清新城疫抗体滴度均显著高于对照组和林可霉素组(P<0.05)；（3）添加甘露寡糖，可显著提高盲肠乳酸杆菌的数量，降低沙门氏菌的数量(P<0.05)，大肠杆菌的数量有增加的趋势但差异不明显(P>0.05)；（4）甘露寡糖可替代林可霉素在三黄鸡养殖中的应用。

Effects of Konjac Mannose-oligosaccharides in Diets on Immunity Function and Mecum Microflora of Three-yellow Broilers

To study the effects of konjac mannose-oligosaccharides in diet on the immunity function and mecum microflora of‘three-yellow broilers’and the possibilitity of substitutes for antibiotics, two hundred sixteen one-day‘three-yellow broilers’were divided randomly into 6 treatments with three replicates. There were twelve broilers per replicate with feed and water provided ad libitum. The basic diet of the six groups was the same and treatments are as follows： control （no MOS and no lincomycin）, 600 mg/kg lincomycin, 1 g/kg MOS, 2 g/kg MOS, 4 g/kg MOS and 8 g/kg MOS. The results showed： （1） 4 g/kg MOS level of the diet significantly improves the index of thymus （P 0.05）, and had a tendency of increasing the index of spleen and bursa but not significantly（P 0.05）. （2） 600 mg/kg lincomycin and 4 g/kg MOS of the diet both increased serum lysozyme level significantly（P 0.05）, however, 8 g/kg MOS of the diet had the higher serum antibody titres to new castle disease virus than the control group and lincomycin group （P 0.05）. （3） Compared to the control group, MOS significantly increased the concentration of lactobacillus and decreased the number of salmonellae （P 0.05）, in addition the number of E. coli had a higher tendency but not significantly（P 0.05）. （4） MOS could substitute for the lincomycin in the‘three-yellow broilers’feeding.