| 葡萄病毒AMP基因RNA干扰载体的构建 |
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| 引用本文： | 丁艳杰 周宗山 董雅凤 徐成楠 吴玉星 迟福梅. 葡萄病毒AMP基因RNA干扰载体的构建[J]. 中国农学通报, 2011, 27(10): 256-259 |
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| 作者姓名： | 丁艳杰 周宗山 董雅凤 徐成楠 吴玉星 迟福梅 |
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| 作者单位： | 中国农业科学院果树研究所,辽宁兴城,125100 |
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| 基金项目： | 现代农业产业技术体系专项资金资助，农业部公益性行业科研专项 |
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| 摘 要： | RNA干扰（RNAi）介导的植物抗病毒研究是近年来引起广泛关注的一项植物抗病毒基因工程策略。本文以GVA运动蛋白（MP）基因为模板扩增出了418 bp的基因片段，根据RNA干扰的基本原理，将目的片段正反向分别插入到载体pFGC5941内含子的两侧以便其转录过程中高效产生RNA发夹结构。经PCR扩增证明已成功构建以GVA MP基因为靶标的干扰载体pFGC5941-GVAFR，并成功转入农杆菌EHA105中，为后续探讨干扰载体的干涉效果奠定了基础。
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| 关 键 词： | 水稻 4001 多芽 突变体 水稻 4001 多芽 突变体 |
| 收稿时间： | 2011-01-12 |
| 修稿时间： | 2011-02-23 |
Construction of the RNAi Vector of GVA MP Gene |
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| Ding Yanjie,Zhou Zongshan,Dong Yafeng,Xu Chengnan,Wu Yuxing,Chi Fumei. Construction of the RNAi Vector of GVA MP Gene[J]. Chinese Agricultural Science Bulletin, 2011, 27(10): 256-259 |
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| Authors: | Ding Yanjie Zhou Zongshan Dong Yafeng Xu Chengnan Wu Yuxing Chi Fumei |
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| Affiliation: | Ding Yanjie,Zhou Zongshan,Dong Yafeng,Xu Chengnan,Wu Yuxing,Chi Fumei(Research Institute of Pomology,CAAS,Xingcheng Liaoning 125100) |
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| Abstract: | The resistance induced by RNAi to plant virus is a new strategy for controlling the viruses in recent years.In this study,according to the basic principles of RNA interference,two fragments(418 bp)ofGVA MP gene were amplified with PCR and inserted into both sides of intron of pFGC5941 in forwards and reverse way so that it produces hairpin RNA during translation efficiently.The construction of the interfering vector pFGC5941-GVAFR targeting toGVA MP gene was identified by PCR,and the vector was transferred ... |
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| Keywords: | grapevive virus A MP gene RNA interference vector construction |
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