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马尾松嫩茎愈伤组织保持、增殖与不定芽分化培养
引用本文:杨模华,李志辉,张冬林,黄振,丁贵杰.马尾松嫩茎愈伤组织保持、增殖与不定芽分化培养[J].中国农学通报,2011,27(10):12-17.
作者姓名:杨模华  李志辉  张冬林  黄振  丁贵杰
作者单位:1. 中南林业科技大学林学院,长沙,410004
2. 中南林业科技大学林学院,长沙410004;缅因大学植物、土壤和环境科学系,美国澳元露04469
3. 贵州大学林学院,贵阳,550025
基金项目:湖南省教育厅优秀青年基金,高等学校博士点科研基金,国家"十一五"林业科技支撑计划项目,湖南省教育厅重点项目
摘    要:笔者以马尾松嫩茎诱导产生的愈伤组织为材料,针对愈伤组织保持、增殖和分化中容易出现褐化死亡的现象,探讨培养基中改变肌醇浓度引起的渗透压变化,调节激素配比和改变培养环境对愈伤组织保持、增殖和丛芽分化的影响。结果表明:(1)改良GD培养基,添加肌醇3.0 g/L,能有效地解决在愈伤组织增殖培养中的褐化问题;(2)在DCR培养基中添加KT 1.0~2.0 mg/L和NAA 0.5 mg/L能逐渐把增殖培养的马尾松愈伤组织从暗培养过渡到光培养,并保持增殖;(3)DCR培养基中添加KT 1.0 mg/L和TDZ 0.5 mg/L可以促进愈伤组织向丛生芽分化,并获得4个丛生芽。本研究结果为马尾松优良基因型快繁中愈伤组织保持、增殖和分化培养提供了研究基础。

关 键 词:霉菌  霉菌  发酵液  玉米  大豆  促长作用  
收稿时间:2010/11/19 0:00:00
修稿时间:2010/11/30 0:00:00

Callus Maintenance, Proliferation and Differentiation of New Sprout of Pinus massoniana L
Yang Mohua,Li Zhihui,Zhang Donglin,Huang Zhen,Ding Guijie.Callus Maintenance, Proliferation and Differentiation of New Sprout of Pinus massoniana L[J].Chinese Agricultural Science Bulletin,2011,27(10):12-17.
Authors:Yang Mohua  Li Zhihui  Zhang Donglin  Huang Zhen  Ding Guijie
Institution:Yang Mohua1,Li Zhihui1,Zhang Donglin1,2,Huang Zhen1,Ding Guijie3(1College of Forestry,Central South University of Forestry and Technology,Changsha 410004,2Department of Plant,Soil and Environmental Sciences,University of Maine,Orono USA 04469,3College of Forestry,Guizhou University,Guiyang 550025)
Abstract:The author described an adventive bud information from callus by new sproutin vitro propagation of adult Pinus massoniana Lamb.Emphasizing on the callus maintenance,proliferation and differentiation,experiments were conducted to discuss osmotic pressure by inositol,hormone in the medium,as well as culture conditions.The results were as followed:(1)adding inositol 3.0 g/L in the modified GD medium could effectively solve the browning problem and maintaining the callus proliferation;(2)To switch the callus cu...
Keywords:biological technology  Pinus massoniana Lamb  new sprout  callus maintenance  callus proliferation  adventive bud differentiation  
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