| 两种同时提取小麦根腐离蠕孢菌DNA和RNA的方法比较 |
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| 引用本文： | 王珍,崔素萍,崔丽琴,左豫虎.两种同时提取小麦根腐离蠕孢菌DNA和RNA的方法比较[J].中国农学通报,2011,27(15):277-280. |
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| 作者姓名： | 王珍 崔素萍 崔丽琴 左豫虎 |
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| 作者单位： | 1. 黑龙江八一农垦大学,大庆,163319
2. 黑龙江贝因美乳业有限公司,安达,151400 |
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| 基金项目： | 黑龙江省普通高等学校青年学术骨干支持计划项目 |
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| 摘 要： | 获得足够数量和高质量的RNA和DNA是进行分子生物学研究工作的基础。以小麦根腐离蠕孢菌为原料，分别采用溶菌酶法和液氮研磨法破碎真菌细胞壁提取DNA，用琼脂糖凝胶电泳、紫外吸收及聚合酶链式反应（PCR）技术进行核酸完整性和纯度检测。结果表明：两种方法在提取DNA时都同时得到了高质量的RNA；PCR检测结果表明DNA完整性比较高，两种方法得到的DNA质量相当；溶菌酶法中得到的RNA质量优于液氮法。利用溶菌酶破壁法提取小麦根腐离蠕孢菌DNA的提取体系可用于RNA的提取，为小麦根腐离蠕孢菌核酸的DNA和RNA的同时提取提供了一种简便、安全、可行的方法。
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| 关 键 词： | 酸性磷酸酶 酸性磷酸酶 |
| 收稿时间： | 2010/11/23 0:00:00 |
| 修稿时间： | 1/5/2011 12:00:00 AM |
Comparing Two Methods for Simultaneous Extraction of DNA and RNA for Bipolaris sorokinianum |
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| Wang Zhen,Cui Suping,Cui Liqin,Zuo Yuhu.Comparing Two Methods for Simultaneous Extraction of DNA and RNA for Bipolaris sorokinianum[J].Chinese Agricultural Science Bulletin,2011,27(15):277-280. |
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| Authors: | Wang Zhen Cui Suping Cui Liqin Zuo Yuhu |
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| Institution: | Wang Zhen 1,Cui Suping 1,Cui Liqin 2,Zuo Yuhu 1 (1 Heilongjiang Bayi Agricultural University,Daqing 163319,2 Heilongjiang Beingmate Dairy Industry Limited Company,Anda 151400) |
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| Abstract: | Obtaining sufficient quantity and high quality of RNA and DNA is the base for carrying out researches in molecular biology.DNA from Bipolaris sorokiniana was attracted by two cell wall lysis methods using lysozyme and liquid nitrogen grinding methods,respectively.Integrality and purity of nucleic acids were detected with agarose gel electrophoresis,ultraviolet absorption and PCR.Ideal results were obtained,when extracting DNA,high quality RNA was obtained at the same time,by using both methods.The results o... |
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| Keywords: | Bipolaris Sorokinianum Nucleic acid lysozyme extraction method PCR |
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