| 一种改良的快速大量提取禾谷类作物成熟籽粒总RNA的方法 |
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| 引用本文： | 高美娟,刘宗才,李丹丹,阚云超,刘卫群.一种改良的快速大量提取禾谷类作物成熟籽粒总RNA的方法[J].中国农学通报,2011,27(7):78-82. |
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| 作者姓名： | 高美娟 刘宗才 李丹丹 阚云超 刘卫群 |
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| 作者单位： | 1. 河南农业大学生命科学学院,郑州,450002
2. 南阳师范学院河南省伏牛山昆虫生物学重点实验室,河南南阳,473061 |
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| 基金项目： | 973国家重大科技专项 |
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| 摘 要： | 禾谷类作物成熟籽粒富含多糖、蛋白质和脂类等成分，传统的提取方法所得的RNA大多不能满足下游操作的需求。此实验利用一种改良的CTAB法对小麦、水稻、玉米成熟籽粒总RNA进行提取，实验步骤简单。RNA得率高，完整性好，电泳条带清晰，OD260/OD280值在1.6~1.8之间。RT-PCR和Northern印迹分析显示该法所提RNA能够满足下游实验操作的要求。进一步分析发现，这种改良的CTAB法对玉米籽粒RNA的提取效果优于小麦和水稻，更适于玉米籽粒RNA的提取。
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| 关 键 词： | ATP酶活性 ATP酶活性 |
| 收稿时间： | 2010/12/13 0:00:00 |
| 修稿时间： | 2011/1/11 0:00:00 |
An Improved Method for Total RNA Extraction from Mature Seeds of Gramineous Grain Crops |
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| Gao Meijuan,Liu Zongcai,Li Dandan,Kan Yuncao,Liu Weiqun.An Improved Method for Total RNA Extraction from Mature Seeds of Gramineous Grain Crops[J].Chinese Agricultural Science Bulletin,2011,27(7):78-82. |
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| Authors: | Gao Meijuan Liu Zongcai Li Dandan Kan Yuncao Liu Weiqun |
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| Institution: | Gao Meijuan 1,Liu Zongcai 2,Li Dandan 2,Kan Yuncao 2,Liu Weiqun 1 (1 Life Science College,Henan Agricultural University,Zhengzhou 450002,2 Henan Key Laboratory of Insect Biology,in Funiu moutain,Nanyang Normal University,Nanyang 473061) |
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| Abstract: | Conventional methods for RNA extraction are not feasible to mature seeds of gramineous crops for the enrichment of polysaccharide,protein and lipid.In this paper an improved method for isolating total RNA from the seeds of wheat,rice and maize is introduced.Comparing with the Conventional methods for RNA extraction,the improved method was of high purity (with the ratio of OD 260 /OD 280 1.6~1.8) and integrity and the results of RT-PCR and Northern blot showed that the RNA extracted by this improved method c... |
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| Keywords: | improved CTAB method RNA extraction grain seed polysaccharide |
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