| 从非变性聚丙烯酰胺凝胶中回收DNA小片段的两种方法比较 |
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| 引用本文： | 章蕾 袁玲 黄建安 吴文亮 陈东生. 从非变性聚丙烯酰胺凝胶中回收DNA小片段的两种方法比较[J]. 中国农学通报, 2011, 27(7): 227-230 |
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| 作者姓名： | 章蕾 袁玲 黄建安 吴文亮 陈东生 |
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| 作者单位： | 湖南农业大学茶学教育部重点实验室/国家植物功能成分利用工程技术研究中心,长沙,410128 |
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| 基金项目： | 国家自然科学基金，湖南农业大学人才稳定基金 |
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| 摘 要： | 从聚丙烯酰胺凝胶中回收DNA片段是分子生物学中的常用技术，尤其针对于DNA小片段的回收。本试验对比了两种从非变性聚丙烯酰胺凝胶中回收DNA小片段的方法（煮沸法和水浴法），并用无水乙醇和3 mol/L醋酸钠共沉淀纯化DNA小片段。采用聚丙烯酰胺凝胶电泳检测回收结果，通过紫外检测进行定量分析，结果显示：水浴法比煮沸法的回收率高。水浴法结合乙醇沉淀法还具有经济和操作简单的优点，可以广泛应用。
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| 关 键 词： | 灰色预测 灰色预测 GM(1 1)模型 人口老龄化 青岛市 |
| 收稿时间： | 2011-01-04 |
| 修稿时间： | 2011-02-18 |
Comparative Study on Two Methods of Recycling Short DNA Fragments from Nondenaturing Polyacrylamide Gel |
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| Zhang Lei,Yuan Ling,Huang Jianan,Wu Wenliang,Chen Dongsheng,Liu Zhonghua. Comparative Study on Two Methods of Recycling Short DNA Fragments from Nondenaturing Polyacrylamide Gel[J]. Chinese Agricultural Science Bulletin, 2011, 27(7): 227-230 |
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| Authors: | Zhang Lei Yuan Ling Huang Jianan Wu Wenliang Chen Dongsheng Liu Zhonghua |
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| Affiliation: | Zhang Lei,Yuan Ling,Huang Jian ' an,Wu Wenliang,Chen Dongsheng,Liu Zhonghua (Key Lab of Tea Science of Ministry of Education,Hunan Agricultural University/National Research Center of Engineering & Technology for Utilization of Functional Ingredients from Botanical,Changsha 410128) |
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| Abstract: | Recycling DNA fragments from polyacrylamide gel is a common technique in molecular biology especially for recycling DNA short fragments.In this experiment,two methods (boiling method and water-bath method) were used to extract short DNA fragments which were co-precipitated with absolute ethanol and 3 mol/ L sodium acetate together.The results were compared by polyacrylamide gel electrophoresis.At last,short DNA fragments was analyzed by the method of ultraviolet absorption.It showed that the recovery rate o... |
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| Keywords: | nondenaturing polyacrylamide gel short DNA fragments recovery purification |
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