| 利用SSR标记技术鉴定玉米品种真实性的研究 |
| |
| 引用本文： | 岳静,朱志成,申雅娟,张卉,王汝宝.利用SSR标记技术鉴定玉米品种真实性的研究[J].中国农学通报,2011,27(12):201-204. |
| |
| 作者姓名： | 岳静 朱志成 申雅娟 张卉 王汝宝 |
| |
| 作者单位： | 1. 沈阳化工大学环境与生物工程学院,沈阳,110142
2. 辽宁省种子管理局,沈阳,110034 |
| |
| 摘 要： | 从20对SSR基本引物中筛选出核心引物，并对辽宁省主推玉米品种进行真实性研究。利用SSR标记技术，选取均匀覆盖玉米染色体组的20对SSR引物对玉米品种进行PCR扩增分析。依据带型清晰与否、稳定性等筛选出14对核心引物用于辽宁省主推玉米品种的真实性鉴定，其中以引物bnlg161k8的多态性最好，且仅采用bnlg161k8、bnlg2305k4及umc1705w1三对引物即可以对实验中所选玉米品种进行真实性鉴定。bnlg161k8、bnlg2305k4及umc1705w1这三对引物可作为玉米品种真实性鉴定的首选引物，既节约成本，又提高效率。
|
| 关 键 词： | 常温贮藏 常温贮藏 |
| 收稿时间： | 2010/12/17 0:00:00 |
| 修稿时间： | 2/9/2011 12:00:00 AM |
Study on Identifying the Authenticity of Maize Varieties by SSR Markers |
| |
| Yue Jing,Zhu Zhicheng,Shen Yajuan,Zhang Hui,Wang Rubao.Study on Identifying the Authenticity of Maize Varieties by SSR Markers[J].Chinese Agricultural Science Bulletin,2011,27(12):201-204. |
| |
| Authors: | Yue Jing Zhu Zhicheng Shen Yajuan Zhang Hui Wang Rubao |
| |
| Institution: | Yue Jing 1,Zhu Zhicheng 2,Shen Yajuan 2,Zhang Hui 1,Wang Rubao 2(1 Shenyang University of Chemical Technology,Shenyang 110142,2 Seeds Administration Bureau of Liaoning Province,Shenyang 110034) |
| |
| Abstract: | In this study,the core primers were selected from 20 pairs of SSR primers to identify the authenticity of maize varieties in Liaoning Province.PCR amplified products of 10 maize hybrids were detected by 20 basic primer pairs averagely distributed on 10 chromosomes of maize by using SSR markers.14 pairs of core primers were selected to identify the authenticity of maize varieties on electrophoresis maps and the stability,et al.Among all the core primer pairs,bnlg161k8 was the best on polymorphism and the aut... |
| |
| Keywords: | maize (Zea mays L ) simple sequence repeat marker core primers authenticity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
