抗烟草黑胫病菌的菌株ZY-19-2的鉴定及发酵条件研究

烟草黑胫病是烟草上重要毁灭性病害之一，生产上主要采取综合防治措施，包括种植抗病品种、农业防治、病害发生期的药剂防治，以及生物防治和植物诱导抗性的利用等，后两者已成为当前综合防治的研究重点。生物防治是烟草黑胫病防治最主要的手段之一。从烟草根际土样中分离、筛选得到一株对烟草黑胫病菌有抑制作用的菌株ZY-19-2，为了评价该菌株利用价值，对菌株ZY-19-2进行了鉴定及不同培养条件下该菌株产几丁质酶的活力。结果表明：该菌株为淡紫拟青霉（Paecilomyces lilacinus）；该菌株的最佳发酵条件以1.2%胶体几丁质为碳源，发酵液初始pH值为6.0，以1%蛋白胨为氮源，0.1%吐温80作为表面活性剂，发酵时间为60 h，接种量为1%，摇床转速为120 r/min，最高酶活达到0.216 U/mL。通过对菌株ZY-19-2发酵条件的优化，为应用该菌株规模化生产高效廉价的几丁质酶、几丁质寡糖及对烟草黑胫病防治奠定基础。

Study on Identification and Fermentation Condition of the Strain ZY-19-2 which Inhibit to Tobacco Black Shank

Tobacco black shank is one of the most serious diseases of tobacco.People mainly adopt integrated management to the disease like,breeding for resistance varieties,agro-protection,chemical control,biological control and plant induced resistance in production of tobacco.Biological control is the best way against the disease for tobacco.The strain ZY-19-2 was isolated and screened from rhizosphere of tobacco,which demonstrated high inhibitory to tobacco black shank.In order to appraise its value,we identified ...