A hypersensitive response was induced by virulent bacteria in transgenic tobacco plants overexpressing a plant ferredoxin-like protein (PFLP) |
| |
| Institution: | 1. Institute of Botany, Academia Sinica, Nankang, Taipei 115, Taiwan, ROC;2. Department of Pathology and Microbiology, National Taiwan University Taipei 106, Taiwan, ROC;3. Department of Life Science, National University of Kaohsiung, Kaohsiung 811, Taiwan, ROC;1. Institute of Biology, Pedagogical University, Podchorążych 2, 30-084 Kraków, Poland;2. Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Łódź, Poland;3. Institute of Environmental Science, Jagiellonian University, Gronostajowa 7a, 30-387 Kraków, Poland;4. Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland;5. Julius Kühn Institute, Federal Research Institute for Cultivated Plants (JKI), Heinrichstraße 243, D-64287 Darmstadt, Germany;6. Małopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, 30-387 Kraków, Poland;1. Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137 Brno, Czechia;2. Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czechia;3. National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czechia |
| |
| Abstract: | The hypersensitive response (HR) displayed by resistance plants against invading pathogens is a prominent feature of an incompatible plant pathogen interaction. It has been shown that tobacco cell cultures transgenic for a plant ferredoxin-like protein (PFLP) that functions as an electron acceptor of Photosystem I increased harpin-mediate HR. In this work we report increased bacterial disease resistance of pflp transgenic tobacco. Compared to the controls, four distinctive characteristics were found in the pflp-transgenics after inoculation with virulent bacterial cells Erwinia carotovora subsp. carotovora and Pseudomonas syringae pv. tabaci: (i) instead of typical disease symptoms, an HR-like necrosis was observed; (ii) the proliferation of the virulent pathogen was highly retarded; (iii) the expression of hsr203j, an HR marker gene, was apparently induced; (iv)H2O2 accumulation was induced immediately. Together, those results demonstrate that enhanced production of PFLP in the transgenic plant conditions the induction of a hypersensitive response during compatible pathogen attack. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
