Preliminary comparisons of a unique freezing technology to traditional cryopreservation methodology of equine spermatozoa |
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| Institution: | 1. Department of Animal and Food Science, Texas Tech University, Lubbock, Texas, USA;2. Department of Obstetrics and Gynecology, Texas Tech University Health Sciences Center, Lubbock, Texas, USA;1. Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018 Hohhot, China;2. Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018 Hohhot, China;3. Inner Mongolia of Agricultural & Animal Husbandry Science, No.22, Zhaojun Road, Yuquan District, 010031 Hohhot, China;1. College of Veterinary Medicine, China Agricultural University, Beijing, China;2. Key Laboratory of Clinical Diagnosis and Treatment Techniques of Animal Disease for Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, China;1. Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China;2. School of Public Health, 014060, Bao Tou Medicine College, Bao Tou, China;3. Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, 010018, Hohhot, China;1. FertileSafe, 11 Haharash Str., Ness Ziona 7403118, Israel;2. Department of Reproduction Management, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, 10315 Berlin, Germany;1. College of Biotechnology, U.P. Pandit Deendayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India;2. Department of Veterinary Physiology, U.P. Pandit Deendayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India;3. Department of Biochemistry, U.P. Pandit Deendayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India;4. College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India |
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| Abstract: | With recent large-breed organization acceptance, the use of frozen semen is gaining more attention in the equine industry. However, cryopreserved stallion semen is commonly associated with poor quality and decreased pregnancy rates as compared with those produced during normal mating or with cooled semen techniques. Therefore our objective was to investigate a new unique freezing technique (UFT) with the intent of improving fertility outcomes. A series of experiments tested the UFT compared with traditional liquid nitrogen methodology in combination with influence of the extenders and stallions used. In Experiment 1, post-thaw motility results of UFT variations were compared with those from liquid nitrogen methods. The averaged post-thaw motility percentages of the 4 UFT treatments were similar when compared with the traditional liquid nitrogen control group (P = .845). In Experiment 2, 2 egg-yolk–based freezing extenders, Biladyl AB intended for bovine samples and Freezing Medium Test Yolk Buffer used for human samples, were compared. A significant difference in the average post-thaw motilities was found between Biladyl AB (17%) and Freezing Medium Test Yolk Buffer (25%) (P < .002). In the third experiment, we compared variability among stallions using the UFT with the intention of creating a more consistent outcome. Post-thaw motilities and percent of original motility returns among the 4 stallions were significantly different (P < .001). In a field trial using shipped semen from a regional stallion station, the UFT demonstrated very promising results.In conclusion, the UFT may potentially be used as an alterative freezing method to replace current liquid nitrogen methodology. However, further investigation is needed to refine techniques. |
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