|
|||||
|
|
| 无溶剂体系脂肪酶催化合成阿魏酸双油酸甘油酯 | |
| 引用本文： | 张蕾,辛嘉英,陈林林,张颖鑫,夏春谷.无溶剂体系脂肪酶催化合成阿魏酸双油酸甘油酯[J].农产品加工．学刊,2008(7). |
| 作者姓名： | 张蕾 辛嘉英 陈林林 张颖鑫 夏春谷 |
| 作者单位： | 1. 哈尔滨商业大学食品科学与工程省重点实验室,黑龙江哈尔滨,150076 2. 哈尔滨商业大学食品科学与工程省重点实验室,黑龙江哈尔滨,150076;中国科学院兰州化学物理研究所羰基合成和选择氧化国家重点实验室,甘肃兰州,730000 3. 中国科学院兰州化学物理研究所羰基合成和选择氧化国家重点实验室,甘肃兰州,730000 |
| 基金项目： | 教育部新世纪优秀人才支持计划(NCET-05-0356); 龙江学者特聘教授计划; 哈尔滨市科学基金项目(2004AFXXJ031) |
| 摘 要： | 对无溶剂体系中脂肪酶催化合成新型油脂抗氧化剂阿魏酸双油酸甘油酯进行了研究。以Novozym 435脂肪酶为催化剂,以阿魏酸乙酯和三油酸甘油酯为底物,合成阿魏酸双油酸甘油酯。探讨了减压旋转蒸发、底物比、温度、反应时间、酶的稳定性等因素对产率的影响。结果表明,无溶剂体系中,在110mg Novozym 435催化下,水活度小于0.01,转速为180r/min,底物比(阿魏酸乙酯∶三油酸甘油酯)为1∶2,温度为50℃,真空度为0.001MPa,旋转蒸发反应120h时的产率最高。固定化酶可反复使用多次,活性没有明显损失。 |
| 关 键 词： | 阿魏酸乙酯 三油酸甘油酯 阿魏酸双油酸甘油酯 Novozym 435脂肪酶 旋转蒸发 |
Lipase-Catalyzed Synthesis of Ferulyl Oleins in Solvent-free Medium |
|
| Zhaag Lei,Xin Jiaying,Chen Linlin,Zhang Yingxin,Xia Chungu.Lipase-Catalyzed Synthesis of Ferulyl Oleins in Solvent-free Medium[J].Nongchanpin Jlagong.Xuekan,2008(7). | |
| Authors: | Zhaag Lei Xin Jiaying Chen Linlin Zhang Yingxin Xia Chungu |
| Abstract: | Lipase-Catalyzed Synthesis is a new- type of antioxidant in free-solvent system was studied. Using Novozym 435 as catalyst,transesterification of ethyl ferulate(EF)with triolein(TO)to form ferulyl oleins in solvent-free medium was investigated. This enzyme was studied on the effects of subs trate ratio,reaction temperature,reaction time,the stability of enzyme. The results showed that the yield of FDO was maximum in free solvent at 50 ℃ after 120 h under optimal reaction conditions which were:water activity(aw)<0.01,molar ration of subs trates is 1∶2,110 mg novozym 435,180 r/min agitation,0.001 Mpa. The enzyme can be reused several times without significant loss of activity. |
| Keywords: | ethyl ferulate triolein ferulyl olein novozym 435 lipase vacuum-rotary evaporation procedure |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |