迟缓爱德华氏菌胞外产物的细胞毒性和动物致病性

分析了２８株迟缓受德华氏菌（Ｅdwardwiella tarda,Ｅt）胞外产物（extracellular puodrcts,ＥＣＰ）的致病性。将Ｅt用覆有玻璃纸的ＴＳＡ培养，１８株（６９．２３％）的 ＥＣＰ使ＨＥp－２细胞产生病认，认圆，脱落。这种细胞毒作用能被ＡＴＣＣ１５９４７株的ＥＣＰ抗体作为探 针进行免疫转印分析，结果显示，不同来源的９株Ｅt的ＥＣＰ在 转印膜上出现３条共同蛋白条带，相对分子质量分别约１４４００、３２、３００、７９、

Cytotoxity and Pathogenicity in the Mice and Fish of Extracellular Products of Edwardsiella Tarda

The cytotoxity ofextracellular products (ECP) of Edwardsiella tarda (Et) strains from different sourceswere investigated. E CP were prepared by a cellophane-overlaid agar method. The ECP of18/26 Et stra ins had a cytotoxic effect on HEp-2 cells, which could be inhibitied by theant iserum against ECP of ATCC15947. The ECP had no toxic effects to HEp-2 cells af terbeing dealt with trypsin or heat. Western-blot of ECP probed with antisera against ECP ofATCC15947 strain showed that 9 virulent strains from different so urces had the same threeantigenic proteins of 14 400, 32 300 and 79 400. The to xity of ECP of 5 selected Etstrains was tested in the mice and fish (Xiphopho rus helleri) with the pathogenicity ofcytotoxic ECP of 4 strains to mice and fish to be 75% and 67.5% respectively, while theECP without cytotoxity had no v irulence to these animals.