PP333对怀山药试管苗生长及生理特性的影响

研究了PP333对怀山药试管苗生长及一些生理指标的影响,结果表明,一定浓度范围内的PP333可使植株矮小,茎粗壮,根数和根长增加,同时也可使试管苗叶片中叶绿素和可溶性蛋白含量、POD活力提高。因此,适宜浓度的PP333可以改变怀山药试管苗的生理特性,达到培育壮苗的目的。其中,铁棍山药和太谷山药的最佳PP333浓度为8 mg/L,47号山药、沙滩圆、济宁牛腿米、肿脖温和栾川野山药为5 mg/L,B号山药为2.5 mg/L。

Effects of PP333 on the Morphological and Physiological Characteristics of Dioscorea opposita Thunb Plantlets

The effects of PP_(333) on the morphological and physiological characteristics of Dioscorea opposita Thunb plantlets were investigated by single factor design,Coomassie Brillian Blue G-250 colorimetry, Guaiacol method and alcohol-acetone distill method.The results were as follows: PP_(333) in a given concentration range could make the plantlets dwarf and robust,the number and length of roots increase;at the same time,PP_(333) also increased the content of soluble protein and chlorophyll,and POD activity of the leaves.It could be concluded that the suitable concentration of PP_(333) can improve the physiological characteristics of Dioscorea opposita Thunb plantlet;the suitable concentrations of PP_(333) for different cultivars are the followings: 8 mg/L for Tiegun and Taig,5 mg/L for No.47,Shatanyuan,Jiningniutuimi,wild Zhongbowen and wild Luanchuan,2.5 mg/L for No.B.