| Agrobacterium tumefaciens-mediated GUS gene transformation of Robinia pseudoacacia ''''Idaho'''' |
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| 引用本文： | Sun Hai-jun Li Min Chen Shou-yi He Si-jie Wang Hua-fang College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,P. R. China Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,P. R. China. Agrobacterium tumefaciens-mediated GUS gene transformation of Robinia pseudoacacia ''''Idaho''''[J]. 中国林学(英文版), 2006, 8(4) |
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| 作者姓名： | Sun Hai-jun Li Min Chen Shou-yi He Si-jie Wang Hua-fang College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 P. R. China Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 P. R. China |
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| 作者单位： | Sun Hai-jun Li Min Chen Shou-yi He Si-jie Wang Hua-fang College of Biological Sciences and Biotechnology，Beijing Forestry University，Beijing 100083，P. R. China Institute of Genetics and Developmental Biology，Chinese Academy of Sciences，Beijing 100101，P. R. China |
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| 基金项目： | 国家高技术研究发展计划(863计划);国家自然科学基金 |
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| 摘 要： | Based on the plant regeneration system, a GUS gene transformation system to Idaho locust (Robinia pseudoacacia 'Idaho') mediated by Agrobacterium tumefaciens was established. The successful transformation was confirmed by regenerating the shoots from the infected leaves in the presence of hygromysin; by histochemical X-gluc assays ofβ-glucuronidase (GUS) and by PCR and PCR-Southern blotting analysis. The ratio of positive transgenic plants is 5.8% (5 out of 86 plants). With this system, the target gene DREB was introduced into the leaves of Idaho locust. The transgenic plants regenerated, which was verified by PCR-Southern blotting. It is suggested that the transformation system could be a new, simple, reliable and practical route to gene transformation of R. pseudoacacia 'Idaho' mediated with A. tumefaciens.
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Agrobacterium tumefaciens-mediated GUS gene transformation of Robinia pseudoacacia ''''Idaho'''' |
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| Sun Hai-jun,Li Min,Chen Shou-yi,He Si-jie,Wang Hua-fang. Agrobacterium tumefaciens-mediated GUS gene transformation of Robinia pseudoacacia ''''Idaho''''[J]. Forestry Studies in China, 2006, 8(4) |
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| Authors: | Sun Hai-jun Li Min Chen Shou-yi He Si-jie Wang Hua-fang |
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| Abstract: | Based on the plant regeneration system, a GUS gene transformation system to Idaho locust (Robinia pseudoacacia 'Idaho')mediated by Agrobacterium tumefaciens was established. The successful transformation was confirmed by regenerating the shoots from the infected leaves in the presence of hygromysin; by histochemical X-gluc assays of β-glucuronidase (GUS) and by PCR and PCR-Southern blotting analysis. The ratio of positive transgenic plants is 5.8% (5 out of 86 plants). With this system, the target gene DREB was introduced into the leaves of Idaho locust. The transgenic plants regenerated, which was verified by PCR-Southem blotting. It is suggested that the transformation system could be a new, simple, reliable and practical route to gene transformation of R.pseudoacacia 'Idaho' mediated with A. tumefaciens. |
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| Keywords: | Robinia pseudoacacia 'Idaho' Agrobacterium tumefaciens GUS gene transformation |
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