| 酵母多糖对小鼠免疫功能影响的体外实验研究 |
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| 引用本文： | 朱延军,刘洪梅,边洪荣,史相国,李树义,张庆波,马志红.酵母多糖对小鼠免疫功能影响的体外实验研究[J].安徽农业科学,2009,37(28):13636-13637. |
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| 作者姓名： | 朱延军 刘洪梅 边洪荣 史相国 李树义 张庆波 马志红 |
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| 作者单位： | 华北煤炭医学院生物科学系,河北唐山,063000 |
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| 基金项目： | 唐山市科学技术研究与发展计划 |
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| 摘 要： | 目的]研究酵母多糖对小鼠免疫功能的影响，为酵母多糖相关产品的开发利用提供可靠的实验依据。方法]分离提取小鼠的脾细胞，分为添加酵母多糖组和不加酵母多糖的对照组，检测小鼠脾细胞的增殖活性；留取细胞上清液，测定白细胞介素2（IL-2）、γ干扰素（IFN-γ）含量；用流式细胞术检测T淋巴细胞和B淋巴细胞的数量，观察酵母多糖对T、B细胞增殖的影响。结果]酵母多糖组增殖活性明显高于对照组（P〈0．01）；在一定剂量范围内，该组IL-2、IFN-γ的含量与酵母多糖呈剂量依赖关系，另外酵母多糖对T、B细胞均有明显的增殖效果。结论]酵母多糖可显著提高小鼠免疫系统的功能。
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| 关 键 词： | 酵母多糖 淋巴细胞 IFN-γ IL-2 |
Study on the Effect of Yeast Polysaccharides on Immune Function of Mice in Vitro |
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| ZHU Yan-jun et al.Study on the Effect of Yeast Polysaccharides on Immune Function of Mice in Vitro[J].Journal of Anhui Agricultural Sciences,2009,37(28):13636-13637. |
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| Authors: | ZHU Yan-jun |
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| Institution: | ZHU Yan-jun et al(Department of Biological Sciences,North China Coal Medical University,Tangshan,Hebei 063000) |
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| Abstract: | Objective]Effects of yeast polysaccharides(YPS) on mice immune function were researched in order to provide a basis for its development and utilization.Method]Extracted the spleen cells of mice and divided them into two groups,one was YPS group,another was negative control group,detected their proliferation activity;got cell supernatant to detect IL-2,IFN-γ;using flow cytometry to check cell subtypes, observed effects of YPS on proliferation of T and B cells.Result]Compared with the control group,YPS group proliferation activity increased significantly(P<0.01).In some dose range,the level of secreting IL-2,IFN-γfrom immune cell has a dose-dependent relationship with YPS, moreover,YPS could enhance T and B cell's proliferation.Conclusion]YPS can significantly improve the immune function of mice. |
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| Keywords: | IL-2 |
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