The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates |
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Authors: | Tae Jung Kim Jae Il Lee |
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Affiliation: | 1School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.;2College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea. |
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Abstract: | Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently, B. hyodysenteriae and B. pilosicoli revealed different restriction patterns; however, the other three species shared the same pattern. These findings are not consistent with a prior report. Differences in 23S rRNA gene sequences, between two B. murdochii strains, 56-150 and 155-20, were observed. These results indicate that 23S rRNA PCR-RFLP could be used as an identification method for pathogenic Brachyspira spp. (B. hyodysenteriae and B. pilosicoli) as well as an epidemiological tool for characterizing spirochetes isolated from swine. |
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Keywords: | Brachyspira spp. PCR-RFLP 23S rRNA spirochete |
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