Effect of Fasciola gigantica excretory secretory antigen on rat hematological indices |
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Authors: | G. Ganga J. P. Varshney R. L. Sharma |
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Affiliation: | 1Clinical Medicine Laboratory, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122 (U.P.), India.;2Division of Medicine, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122 (U.P.), India.;3Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122 (U.P.), India. |
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Abstract: | The present study was undertaken to investigate the effect of Fasciola gigantica excretory secretory antigen (Fg-ESA) on rat hematological indices. Fg-ESA was prepared by keeping thoroughly washed 40 F. gigantica flukes in 100 ml phosphate buffer saline (PBS) for 2 h at 37℃, and centrifuging the supernatant at 12,000 g at 4℃ for 30 min. The protein content of Fg-ESA was adjusted to 1.8 mg/ml. The rats were randomly divided into two groups of six rats each. Rats in group A received 0.5 ml of Fg-ESA intraperitoneally (i.p.) for 7 days, whereas control rats in group B received 0.5 ml of PBS i.p. for 7 days. Hemograms of both groups were studied initially and on days 0, 2, 4, 14 and 21 after the final injection of Fg-ESA or PBS. Progressive and significant (p < 0.01) declines in the values of hemoglobin, hematocrit, and total erythrocyte count were observed without significant (p > 0.05) changes in the values of mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, or mean corpuscular volume in group A. Thus, we conclude that Fg-ESA induces normocytic normochromic anemia in rats. |
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Keywords: | excretory secretory antigen Fasciola gigantica hematocrit hemoglobin rat total erythrocyte count |
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