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| PRRSV缺失变异毒株JL/07/SW株的分离鉴定及序列分析 | |
| 引用本文： | 李志杰,丁壮,孟轲音,宣华,王贵平,高恩鹏,丛彦龙,母连志. PRRSV缺失变异毒株JL/07/SW株的分离鉴定及序列分析[J]. 中国兽医学报, 2009, 29(7) |
| 作者姓名： | 李志杰 丁壮 孟轲音 宣华 王贵平 高恩鹏 丛彦龙 母连志 |
| 作者单位： | 1. 吉林大学,畜牧兽医学院,吉林,长春,130062 2. 军事医学科学院,军事兽医研究所,吉林,长春,130062 3. 广东省农业科学院,兽医研究所,广东广州,510000 |
| 摘 要： | 分离并鉴定了1株猪繁殖与呼吸综合征病毒,经病毒生物学特性测定、血清学试验、病毒基因鉴定,确定为美洲型猪繁殖与呼吸综合征病毒,将其命名为JL/07/SW株.根据猪繁殖与呼吸综合征病毒VR-2332株及变异毒株的核苷酸序列,设计合成了针对NSP2变异序列、N基因以及GP5基因的引物,扩增出正确的序列,经测定的序列比对后,发现该毒株为美洲型变异毒株,NSP2上缺失了30个氨基酸,而GP5和M基因相对保守. |
| 关 键 词： | 猪繁殖与呼吸综合征病毒 分离 鉴定 序列分析 |
Isolation,identification and sequence analysis of the strain JL/07/SW with deletion mutations of PRRSV |
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| LI Zhi-jie,DING Zhuang,MENG Ke-yin,XUAN Hua,WANG Gui-ping,GAO En-peng,CONG Yan-long,MU Lian-zhi. Isolation,identification and sequence analysis of the strain JL/07/SW with deletion mutations of PRRSV[J]. Chinese Journal of Veterinary Science, 2009, 29(7) | |
| Authors: | LI Zhi-jie DING Zhuang MENG Ke-yin XUAN Hua WANG Gui-ping GAO En-peng CONG Yan-long MU Lian-zhi |
| Abstract: | One strain of PRRSV was isolated from tissue of piglets died of obvious respiratory syndrome and high fever.It was identified as a strain of American type PRRSV by serological test and gene sequencing.According to American type PRRSV VR-2332,we designed 3 pairs of primers to aim directly at N gene,GP5 gene and variation sequence of NSP2.The results of sequence analysis indicated a discontinuous deletion of 30 amino acids in nonstructural protein 2 (NSP2).N and GP5 gene is conservative relatively. |
| Keywords: | PRRSV isolation identification sequence analysis |
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