A simple and efficient method for extraction of PCR-amplifiable DNA from chicken eggshells |
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| Authors: | Kazuhiro RIKIMARU Hideaki TAKAHASHI |
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| Affiliation: | Livestock Experiment Station, Akita Prefectural Agriculture, Forestry and Fisheries Research Center, Daisen, and;Animal Breeding and Reproduction Research Team, National Institute of Livestock and Grassland Science, Tsukuba, Japan |
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| Abstract: | Recently, we reported a method for discriminating a Japanese brand of chicken, the Hinai-jidori. As an application of this method for discriminating Hinai-jidori eggs, we here report an efficient method for extracting maternal DNA from eggshells. Eggshell powder was completely decalcified with EDTA solution, and then DNA was isolated by conventional phenol-chloroform extraction and ethanol precipitation. The efficiency of DNA recovery from eggshells was 50-fold higher than that of a previously reported method. The recovered DNA could be used for PCR, and 10 markers for identifying the Hinai-jidori chicken were detected. The genotypes of the Hinai-jidori exactly matched those of the Hinai-dori breed. Using this method, Hinai-jidori and Hinai-dori eggs could be distinguished from the eggs of Rhode Island Reds. This is the first report of a technique that can be used to discriminate the eggs of Hinai-jidori from those of other chickens, and it can also be utilized to validate the labeling of Hinai-jidori eggs in the market. |
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| Keywords: | brand discrimination chicken egg Hinai-jidori chicken microsatellite markers |
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