麦长管蚜在3个小麦品种上取食行为的EPG比较

 【目的】研究不同抗性水平的小麦品种苗期对麦长管蚜的抗蚜机制。【方法】用EPG技术对麦长管蚜在3个小麦品种苗期的取食行为进行检测。【结果】麦长管蚜在品种Ww2730上口针开始接触叶面时间显著晚于小偃22和Batis，第1次持续刺探前的刺探次数显著多于小偃22和Batis，开始刺入叶面后第1次刺探的持续时间极显著短于小偃22和Batis；在Ww2730和小偃22上的PdⅡ-1的平均时间显著长于Batis；Ww2730上C波阶段2个Pd波间的间隔平均时间显著大于另外2个品种；在Ww2730木质部取食（G波）的次数和时间显著大于其它2个品种；Batis上的点G波的次数极显著地高于Ww2730，而平均持续时间极显著短于小偃22和Ww2730；在Batis和小偃22上E1波的总时间，E1波的最大值、跟随E2波的E1波的平均时间均显著大于Ww2730；3个品种间第1次E1波持续的时间差异不显著,但Ww2730和小偃22上其它E1波持续的时间极显著少于Batis；3个品种上其它波形参数，包括F波的次数和总时间、E2波的次数和总时间没有差异。【结论】Ww2730抗麦长管蚜机制可能在于表皮有取食抑制因子，叶肉细胞壁较厚，韧皮部存在次生物质和(或)营养不平衡。小偃22苗期的抗蚜机制为叶肉部细胞壁较厚，细胞密度大。麦长管蚜适应Ww2730抗性的策略可能是在第2次取食时采取了减少在有毒次生物中的暴露时间。

EPG Comparison of Sitobion avenae(Fab.)Feeding Behaviors on Three Wheat Varieties

【Objective】The object was to study resistance mechanism of 3 different wheat varieties to S. avenae. 【Method】Aphids feeding behavior had been ascertained used EPG technique. 【Results】When this aphids feeding onWw2730 seedlings, time to 1st duration probing late than Xiaoyan22 and Batis significantly, probing interrupt before 1st duration probing more than Xiaoyan22 and Batis significantly, 1st duration probing shorter than Xiaoyan22 and Batis significantly; Duration of pd II-1 on Ww2730 and Xiaoyan22 longer than Batis significantly; Duration of pd in C wave on Ww2730 longer than Xiaoyan22 and Batis significantly; Times and duration of G wave (ingestion in xylem) on Ww2730 more and longer than Xiaoyan22 and Batis significantly; Times of spot G wave on Batis more than Ww2730 significantly, but duration of spot G shorter than Xiaoyan22 and Batis significantly; All time and Max duration of E1 fractions, and time of E1 fractions than following E2 wave period on Xiaoyan22 and Batis were all longer than Ww2730 significantly; there are no different of mean duration of 1st E1 wave on 3 varieties, but other E1 wave mean duration on Ww2730 and Xiaoyan22 shorter than Batis significantly; the other wave parameters, include times and duration of F and E2 were all no different on 3 wheat varieties.【Conclusion】Resistance mechanism of Ww2730 seelding to S. avenae is repress factor to S. avenae feeding in epidermis, thicker cell wall in mesophyll and has secondary metabolites in phloem. Resistance mechanism of Xiaoyan22 seelding to S. avenae is thicker cell wall and more cell layer in mesophyll. The feeding strategy of S. avenae against resistance of ww2730 is shorten second duration of secrete watery salivation to xylem than first.