| CryIA(c)和gna融合基因植物表达载体的构建 |
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| 引用本文： | 冯翠莲,张树珍.CryIA(c)和gna融合基因植物表达载体的构建[J].热带作物学报,2010,31(2):224-228. |
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| 作者姓名： | 冯翠莲 张树珍 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,海口571101;海南大学农学院,海南儋州,571737
2. 中国热带农业科学院热带生物技术研究所,海口,571101 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费，中国热带农业科学院院基金，现代农业产业技术体系建设专项基金 |
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| 摘 要： | 采用重叠延伸PCR技术(gene splicing by overlap extension PCR,简称SOE PCR),通过连接多肽FMDV2A序列将CryIA(c)和gna基因相融合,得到CryIA(c)-2A-gna抗虫融合基因。并进一步将玉米UBI启动子和融合基因(CryIA(c)-2A-gna)分别连接到植物表达载体pCAMBIA3300上,构建成由玉米UBI启动子驱动抗虫融合基因的植物表达载体pNUBG。经过限制性酶切分析鉴定,结果表明,含有融合基因的植物表达载体构建成功。
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| 关 键 词： | 重叠延伸PCR 融合基因 表达载体 |
Construction of Expression Vector Harboring CryIA(c) and gna Fusion Gene |
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| Feng Cuilian and Zhang Shuzhen.Construction of Expression Vector Harboring CryIA(c) and gna Fusion Gene[J].Chinese Journal of Tropical Crops,2010,31(2):224-228. |
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| Authors: | Feng Cuilian and Zhang Shuzhen |
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| Institution: | Feng Cuilian1,2,Zhang Shuzhen1 1 Institute of Tropical Bioscience , Biotechnology,CATAS,Haikou,Hainan 571101,2 College of Agronomy,Hainan University,Danzhou,Hainan 571737,China |
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| Abstract: | CryIA(c)and gna genes were ligated with FMDV2A by using the technique of gene splicing by overlap extension PCR(SOE PCR)to produce an insect-resistant fusion gene CryIA(c)-2A-gna.Then the maize's UBI promoter and the fusion gene were cloned into the plant expression vector pCAMBIA3300 to construct a plant expression vector pNUBG consisting of UBI promoter-driven insect-resistant fusion gene.These recombinant plasmids were confirmed by restriction enzyme and PCR analysis,and the results showed that these rec... |
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| Keywords: | FMDV2A splicing by overlap extension PCR FMDV2A fusion gene expression vector |
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