Single-nucleotide polymorphism identification in the caprine myostatin gene |
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| Authors: | XL Li ZhL Wu YF Gong YQ Liu ZZ Liu XJ Wang TR Xin & Q Ji |
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| Institution: | College of Animal Science and Technology, Hebei Agricultural University, Baoding, China; Department of Animal Science, Hebei Normal University of Science &Technology, Changli, China; College of Life Science, Nankai University, Tianjin, China; School of Medicine, Jishou University, Jishou, China; School of Life Science, Nanchang University, Nanchang, China |
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| Abstract: | Polymerase chain reaction (PCR) products of MSTN gene amplified from 35 goats representing 17 Chinese indigenous goat breeds and five imported goat breeds were sequenced to identify the single‐nucleotide polymorphisms (SNPs) of a 379‐bp fragment including part of intron 2 and exon 3 of MSTN gene. A total of eight SNPs (A1980G, G1981C, A1982G, G1984T, A2121G, T2124C, G2174A and A2246G) were identified among the sequenced goats. The SNPs found are all located in intron 2 except for A2246G, which was a synonymous mutation in exon 3. Four haplotypes were sorted from these eight SNPs, of which, haplotype I (AGAGATGA) and haplotype II (GCGTGTAA) are the two main haplotypes with the frequency of 77.8% and 14.8% respectively. The SNPs found at positions 1980, 1981, 1982, 1984 and 2121 might be linked to inheritance completely. |
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