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绿色木霉和枯草芽孢杆菌对玉竹锐顶镰孢菌的拮抗作用研究
引用本文:杨洪一,周阳阳. 绿色木霉和枯草芽孢杆菌对玉竹锐顶镰孢菌的拮抗作用研究[J]. 黑龙江农业科学, 2010, 0(12): 57-59
作者姓名:杨洪一  周阳阳
作者单位:[1]东北农业大学园艺学院,黑龙江哈尔滨150030 [2]东北林业大学生命学院,黑龙江哈尔滨150040
基金项目:黑龙江省博士后基金资助项目
摘    要:采用平板对峙培养法、对扣培养法和圆盘滤膜法研究绿色木霉和枯草芽孢杆菌对玉竹锐顶镰孢菌的拮抗作用。结果表明:木霉和枯草芽孢杆菌对锐顶镰孢菌菌丝生长具有一定抑制作用,木霉的抑制效果较差,枯草芽孢杆菌的抑制效果较好,其中枯草芽孢杆菌菌株Dc10的抑制作用最强,其非挥发性代谢产物对病原菌的抑制率可达100%。

关 键 词:锐顶镰孢菌  玉竹  木霉  枯草芽孢杆菌

Study on the Antagonistic Activity of Trichoderma viride and Bacillus subtilis against Fusarium acuminatum
YANG Hong-yi,ZHOU Yang-yang. Study on the Antagonistic Activity of Trichoderma viride and Bacillus subtilis against Fusarium acuminatum[J]. Heilongjiang Agricultural Science, 2010, 0(12): 57-59
Authors:YANG Hong-yi  ZHOU Yang-yang
Affiliation:1.Horticultural College of Northeast Agricultural University,Harbin,Heilongjiang 150030;2.Life Science College of Northeast Forestry University,Harbin,Heilongjiang 150040)
Abstract:The antagonistic activity of Trichoderma viride and Bacillus subtilis against Fusarium acuminatum was analyzed by competitive culture,culture on facing each other,and filter membrane culture in disk,respectively.The results indicated that both T.viride and B.subtilis had the efficacy to inhibit the development of mycelium of F.acuminatum.The efficacy of inhibition of T.viride was low,but B.subtilis had strong inhibition capability to F.acuminatum.It was the best for the efficacy of inhibition of B.subtilis Dc10,its inhibition for non-volatile metabolize product could up to 100%.
Keywords:Fusarium acuminatum  Polygonatum odoratum  Trichoderma viride  Bacillus subtilis
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