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| 应用低能离子束将抗菌肽ShivaA基因导入恢复系明恢63的研究(简报) | |
| 引用本文： | 倪大虎,向太和,张盘娣,吴家道,杨剑波,贾士荣,余增亮. 应用低能离子束将抗菌肽ShivaA基因导入恢复系明恢63的研究(简报)[J]. 安徽农业科学, 2000, 28(4): 408-409,419 |
| 作者姓名： | 倪大虎 向太和 张盘娣 吴家道 杨剑波 贾士荣 余增亮 |
| 作者单位： | 1. 安徽省农业科学院水稻研究所,农业部水稻遗传育种重点开放实验室,合肥,230031 2. 中国农业科学院生物技术中心 3. 中国科学院等离子体物理研究所 |
| 摘 要： | 利用低能离子束介导转基因法于 1993年首次获得成功 ,本研究通过这一技术将抗菌肽ShivaA基因导入水稻恢复系明恢6 3成熟胚中 ,经过诱导愈伤和绿苗分化 ,并再生植株 ,经PCR检测分析证明外源基因已整合到再生植株基因组中。 |
| 关 键 词： | 水稻育种 低能离子束介导法 抗茵肽ShivaA 明恢63 |
Study on Transferring Antibacterial Polypetide Shiva A Gene into Rice Restore Line Minghui No 63 by Low Energy Ion Beam |
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| Ni Dahu et al. Study on Transferring Antibacterial Polypetide Shiva A Gene into Rice Restore Line Minghui No 63 by Low Energy Ion Beam[J]. Journal of Anhui Agricultural Sciences, 2000, 28(4): 408-409,419 | |
| Authors: | Ni Dahu et al |
| Affiliation: | Rice Research Institute and Key Laboratory of Rice Genetics and Breeding of Agricultural Ministry Anhui Academy of Agricultural Sciences.Hefei 23001 |
| Abstract: | The antibacterial polypetide Shiva A genes were transfered into mature embryos of restore line Minhui 63 of hybird rice by low energy ion beam and the transgenic plants were obtained.Foreign gene into genomes of transgenic plants was confirmed by PCR assay. |
| Keywords: | Rice breeding Low energy ion beam Antibaterial polypetide Shiva A gene Restore line Minhui63 of hybird ri |
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