不同AM菌根菌分泌的磷酸酶对根际土壤有机磷的影响

以三叶草为材料,利用3室隔网培养方法,研究了4种AM菌根菌侵染三叶草后对根际土壤酸性和碱性磷酸酶活性以及菌丝酶活性对土壤有机P的影响。结果表明,接种AM菌根菌 (9周) 对根际土壤酸性和碱性磷酸酶活性均有增强作用,但作用强度主要取决于菌丝在土壤中的生长状况,Glomus属菌根菌在整个菌丝室 (0~6cm) 都影响土壤磷酸酶的活性,其活性在整个菌丝室中都比Gigaspora的高。同一属不同种的根际土壤磷酸酶活性差异不大。AM菌根根际土壤磷酸酶对土壤有机P的降解有很强的促进作用。

EFFECT OF ARBASCULAR MYCORRHIZA FUNGI (Glomus Mosseae, Glomus Versiformea, Gigaspora Margarita and Gigaspora Rosea) ON PHOSPHATASE ACTIVITIES AND SOIL ORGANIC PHOSPHATE CONTENT IN CLOVER RHIZOSPHERE

In order to study whether there is genetic diversity among AMF in utilizing soil organic phosphate, a pot experiment was carried out to study effect of arbuscular mycorrhizal fungus (Glomus Mosseae, Glomus Versiformea, Gigaspora Margarita and Gigaspora Rosea) on acid and alkaline phosphatase activities and soil phosphate in the rhizosphere of clover. The pot was separated by 30ìm nylon net into three compartments. the central one was for root growth, and the other two for hyphal growth. The two outer compartments were filled with soils collected from field plots either receiving chemical fertilizer or manure in a long-term fertilizer experiment. The treatments consisted of sterilized soil either uninoculated or inoculated with AMF. Soil phosphatase and phosphate were examined, separately, 9 weeks after sowing. The results showed that the activities of acid and alkaline phosphatase were increased with mycorrhizal inocunation. AMF Glomus increased soil phosphatase activity in the whole hyphae compartment. Soil phosphatase activities were higher in the Glomus hyphae compartment than in the Gigaspora hyphae compartment. While there was no difference between AMF of the same genus. Decomposition of organic phosphate compound was promoted by the AMF phosphatase efficiently.