稻瘟病菌群体育性分析的主要影响因子研究

 将稻瘟病菌10个两性可育菌株,包括GUY11、KA3、KA9、KA7、6023、2539W、8773R-19、8773R-27、8113R-2和8113R-10相互交配,结果表明,10个菌株育性好,交配型明确;2539W已丧失雌性功能,不再是两性菌株。原先由6023/2539W测为可育的24个福建省田间稻瘟病菌菌株分别与另外8个两性菌株交配,结果得出不同两性菌株测得同一组稻瘟病菌菌株的育性差异很大,这可能是由于两性菌株的交配能力强弱差异以及两性菌株与待测菌株之间的亲和性不同引起的。分析认为,GUY11和KA3最适于测定稻瘟病菌群体的育性和交配型,并用其测定了227个来自福建省田间稻瘟病菌菌株的育性。结果表明,福建省稻瘟病菌群体可育菌株率为81.1%,其中81.0%为交配型MAT1-2,19.0%为MAT1-1。研究结果将有助于进一步认识稻瘟病菌的群体结构特点及其演化规律。

Factors affecting population fertility analysis of Magnaporthe grisea

10 hermaphroditic isolates including GUY11, KA3, KA9, KA7, 6023, 2539W, 8773R-19, 8773R-27, 8113R-2 and 8113R-10 of Magnaporthe grisea were crossed each other to clarify the cause of difference in fertility analysis conducted by different laboratories. The results showed that 10 isolates were her-maphroditic fertile with clear mating type (either MAT1-1 or MAT1-2) except for strain 2539W, which lost the capability to function as a female. 24 isolates from Fujian rice fields, fertile when mated with 6023/2539W, showed difference in fertility when mated with other 8 hermaphroditic isolates, it might be caused by the difference in compatibility between tested isolates and hermaphrodites. Considering the fertility, compati-bility and host sources, it was suggested that GUY11 and KA3 were the best hermaphroditic isolates in mating type and fertility analysis. Fertility and mating type of 227 isolates from Fujian rice fields were further tested with GUY11 and KA3, the results revealed that 81.1% were fertile with a ratio of 81.0:19.0 for MAT1-2 to MAT1-1. The study is helpful to understand the fungal population genetics and evolution.