IN VITRO TESTING OF 99mTc-LABELED MONOCLONAL ANTIBODIES SPECIFIC FOR DIROFILARIA IMMITIS |
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Authors: | Gregory B Daniel DVM Rodger V Allhands DVM PhD |
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Institution: | Department of Veterinary Biosciences, College of Veterinary Medicine, University of Illinois, Urbana, Illinois. |
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Abstract: | Monoclonal antibodies specific for Dirofilaria immitis were labeled with technetium-99m (99WTc), and their affinity was assessed in vitro. Of six monoclonal antibodies tested, three were IgG and three were IgM. A canine polyclonal IgG was used to establish the labeling technique and later as a control. Diethylenetriaminepentaacetic acid (DTPA) was used as a bifunctional chelator to bind 99mTc covalently to the antibody. Radiochemical purity was measured with the use of gel filtration by a Sephadex G-25 column linked to an absorbance monitor and with instant thin-layer chromatography. The radiochemical purity had no significant influence on percent uptake on the heartworms. The clone designated as Di 130.1 had a significantly higher percent uptake than other clones tested. |
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Keywords: | monoclonal antibodies radioimmunoimaging Dirofilaria immitis 99Tc |
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