5株氟罗沙星单克隆抗体杂交瘤细胞株的品质评价

摘 要：通过对5株杂交瘤细胞株分泌稳定性、抗体亚型、亲和力、特异性等方面的测定，表明其染色体众数为87-90条，具有众数的细胞所占比例为81-84%，单抗亚型均为IgG1型，腹水单抗的亲和常数为108～109M－1，氟罗沙星单抗与诺氟沙星、环丙沙星、恩诺沙星、链霉素、庆大霉素、卡那霉素的交叉反应试验表明。所制备的抗氟罗沙星单抗对氟喹诺酮类药物有较高的交叉反应率。与链霉素、庆大霉素、卡那霉素无交叉反应。

Quality Evaluation on the Monoclonal Antibodies of Against Fleroxacin of Five Hybridoma Cells

Abstract:The quality of the prepared anti-Fleroxacin monoclonal antibodies were evaluated by the stability of secretion, antibody-subtype , affinity and specificity experiments’results. It showed that the five strains of hybridoma cells contained modal number of chromatin for 87-90 which occupied for 81-84%, subtypes of monoclonal antibodies were all IgG1 types, affinity constant of ascites monoclonal antibodys was 108－109M-1.The cross-reaction experiments of Fleroxacin monoclonal antibody were carried out with Norfloxacin,Ciprofloxacin,Enrofloxacin,Sreptomycin,Gentamicin,Kanamycin.From the experiments we know that the prepared anti-fleroxacin monoclonal antibodies had a very high reaction rate with fluoroquinolones, but had no reaction with magnamycin, kanamycin, streptomycin.