| 日本矮生沿阶草愈伤组织的诱导及其分化 |
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| 引用本文： | 李晶,孙吉雄,梁慧敏.日本矮生沿阶草愈伤组织的诱导及其分化[J].草业科学,2009,26(4):150-153. |
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| 作者姓名： | 李晶 孙吉雄 梁慧敏 |
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| 作者单位： | 1. 甘肃农业大学草业学院,甘肃,兰州,730070
2. 江苏农林职业技术学院,江苏,句容,212400 |
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| 摘 要： | 选用不同外植体和不同培养基,对日本矮生沿阶草Ophiopogon japonicus进行了愈伤组织的诱导及分化的研究.结果表明:幼嫩叶片的基部是最适宜诱导愈伤组织的外植体.G3(MS+KT 0.2 mg/L +2,4-D 0.1 mg/L)培养基的愈伤组织的诱导率达到89%以上,将诱导的愈伤组织置于J1(MS+BA 2.0 mg/L+NAA 0.2 mg/L)培养基中的愈伤组织的分化率最高,达到59%.因此,选用幼嫩的叶片基部置于G3培养基进行诱导,继代一次后置于J1培养基进行分化是最有利于再生的途径.
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| 关 键 词： | 外植体 培养基 愈伤组织 诱导率 分化 再生 |
Study on callus induction and plantlet regeneration of Ophiopogon japonicus |
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| LI Jing,SUN Ji-xiong,LIANG Hui-min.Study on callus induction and plantlet regeneration of Ophiopogon japonicus[J].Pratacultural Science,2009,26(4):150-153. |
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| Authors: | LI Jing SUN Ji-xiong LIANG Hui-min |
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| Institution: | 1.College of Pratacultural Science;Gansu Agricultural University;Lanzhou 730070;China;2.Jiangsu Polytechnic College of Agriculture and Forestry;Jurong 212400;China |
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| Abstract: | The differentiation of Ophiopogon japonicus callus from different explants with different medium was conducted. The result showed that the base part of young leaf was best for callus induction. and the induction rate was more than 89% in G3 medium (MS+KT 0.2 mg/L +2,4-D 0.1 mg/L), and the differentiation rate could reach 59% in J1 medium (MS +BA 2.0 mg/L +NAA 0.2 mg/L). |
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| Keywords: | explant medium callus induction differentiation regeneration |
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