东方百合‘索邦’诱导小鳞茎发生过程中的细胞学观察

 以东方百合‘索邦’鳞片为试材，研究鳞片扦插诱导小鳞茎形成的过程及其鳞片内部糖和蛋白质的分布特点。经石蜡切片PAS和汞溴酚兰组织化学染色观察发现：（1）小鳞茎起源于近轴基部8～14层薄壁细胞，为内起源；形态发生过程依次经过了启动、生长锥形成、叶原基形成和小鳞茎形成；小鳞茎的形成与分生细胞团所在位置有关。（2）小鳞茎发生过程中多糖主要以颗粒状存在于分裂旺盛的分生组织和维管组织周围，多糖含量高时有利于诱导形成维管组织；蛋白质主要集中于细胞核周围；多糖和蛋白质在分生组织及其周围细胞之间存在着浓度梯度，由顶端分生组织细胞到周围的薄壁细胞中，蛋白质含量由高到低，多糖含量则由低到高。

Cytological Observation on the Bulblet Induction of Lilium oriental 'Sorbonne'

The initiation and development of the bulbet of Lilium oriental ‘Sorbonne’ were studied by slicing up and histochemistry coloration. It was showed that the bulblet originated from 8-14th layer inner parenchyma cells near the axle of the scale. the development of bulblet could be diveded four stages according to morphogenesis changes. The saccharide was existed in meristematic tissue and vascular organization with mainly grain-like form, which was indicated that high content of saccharide could induce vascular organization, otherwise the protein was mainly distributed around nuclei duriong bulblet induction. The concentration gradient of saccharide and protein. was observed between meristematic tissue and surrounding parenchyma cells with higher saccharide and lower protein in the differentiation tissueand.