The effects of stressful exercise on leukocytes in cattle with experimental pneumonic pasteurellosis |
| |
Authors: | N V Anderson Y D Youanes J G Vestweber C A King R D Klemm G A Kennedy |
| |
Institution: | (1) Department of Surgery and Medicine, College of Veterinary Medicine, Kansas State University, 66506 Manhattan, Kansas, USA;(2) Department of Laboratory Medicine, College of Veterinary Medicine, Kansas State University, 66506 Manhattan, Kansas, USA;(3) Department of Anatomy and Physiology College of Veterinary Medicine, Kansas State University, 66506 Manhattan, Kansas, USA;(4) Department of Veterinary Diagnosis, College of Veterinary Medicine, Kansas State University, 66506 Manhattan, Kansas, USA;(5) Present address: 207 Gardendale Street, 48220 Ferndale, MI, USA |
| |
Abstract: | Seven yearling bulls were treated with stressful exercise and intrabronchial Pasteurella haemolytica A1. Group 1 bulls (nos. 1–4) underwent treadmill exercise and, 24 days later, intrabronchial instillation of P. haemolytica A1. Group 2 bulls (nos. 5–7) underwent treadmill exercise, followed 30 min later by intrabronchial P. haemolytica A1. Blood lactic acid values were raised (p<0.05) by treadmill exercise only, but plasma cortisol was raised (p<0.05) by treadmill exercise and by P. haemolytica A1 infection. Neutrophils in bronchoalveolar lavage (BAL) differed from control values 24 h after treadmill exercise, and 1 h and 4 h after P. haemolytica A1 infection.Respiratory disease was more severe and the gross lung lesions were larger in group 2 bulls than in group 1 bulls. P. haemolytica A1 was recovered from the livers, spleens and mesenteric lymph nodes of group 2 but not group 1 bulls, suggesting that group 2 bulls had experienced bacteraemia. Decreased neutrophils in BAL fluid from group 2 bulls at 1 h and 4 h after infection suggests that exercise transiently inhibited neutrophil egress from the blood to the alveoli; BAL neutrophils peaked at 1 h and 4 h after infection in group 1 bulls but declined at 24 h. We conclude that group 2 bulls were made more susceptible to experimental pneumonic pasteurellosis by stressful exercise.Abbreviations ADCC
antibody dependent, cell-mediated cytotoxicity
- AM
alveolar macrophages
- BAL
bronchoalveolar lavage
- CFU
conlony-forming units |
| |
Keywords: | cattle cortisol exercise lactic acid leukocytes Pasteurella haemolytica stress treadmill |
本文献已被 SpringerLink 等数据库收录! |
|