Effect of Prolactin Isolated from Human Amniotic Fluid on Cultured Bovine Cells of the Reproductive Tract and Splenocytes

Contents: Prolactin (PRL) was isolated from human amniotic fluid and than fractionated on Concanavalin A-Sepharose (ConA-S). Two fractions were tested for the ³H-thymidine incorporation in the DNA of bovine granulosa, oviductal and stromal cells as well as splenocytes. All cell types reacted to PRL I or PRL II in a dose dependent manner. In general the effect of PRL I was greater than PRL II, and low concentrations of both enhanced the cell proliferation more than higher doses. The incorporating effect of ³H-thymidine under the influence of PRL I was higher in stromal cells and splenocytes than in granulosa and oviductal cells. PRL II did not have such a stimulating effect, even the presence of 14 ng/ml resulted in a decrease of ³H-thymidine incorporation in granulosa cells. The reason of this different intensity of tested PRL variants may depend on the molecular heterogeneity. The results indicate that PRL forms possess a cel type-specific effect on the proliferation of cells of the bovine female reproductive tract and splenocytes.